Quantitative Translation of the Genome to Proteome UCSF Stephen and Nancy Grand Multiple Myeloma Translational Initiative

Welcome to the Wiita Lab! We are an experimental research group in the Dept. of Laboratory Medicine at the University of California, San Francisco. Our lab is focused on how changes in the level of proteome govern cellular phenotype, particularly in relation to perturbations in the genome. We are interested in this problem both from the perspective of cancer as well as constitutional genetics.

In general, over the past two decades our knowledge of genomic changes in disease states has greatly expanded. However, any true change in cellular or disease phenotype must be decoded from genomic changes into changes in the proteome, whether at the level of protein abundance, post-translational modification, or protein localization. Understanding and quantifying this decoding of the genome to proteome on a broad scale remains much less understood. In our studies in cancer, we primarily study hematologic malignancies (blood cancers) by using therapeutic perturbation and CRISPR/Cas9 genome engineering. We aim to monitor complex effects of drug- and genome-level perturbation using a combination of global proteome analysis by shotgun mass spectrometry, targeted mass spectrometry methods, chemical enrichment of cell-surface localized and DNA-bound proteins, RNA-seq, and ribosome profiling. In constitutional genetics, we are particularly interested in DNA copy number variants (CNVs), known to cause effects such as cardiac anomalies, autism, and developmental delay. Yet patients with these CNVs can manifest very different phenotypes. We aim to understand whether this "variable penetrance" may result from patient-specific alterations at the level of the proteome.

Our group is also the home of the UCSF Stephen and Nancy Grand Multiple Myeloma Translational Initative Laboratory. In this capacity, we aim to push forward the development and validation of new therapeutic strategies in multiple myeloma, a currently incurable malignancy of plasma cells and the second most common hematologic malignancy in the United States. Our ultimate goal is to use a combination of systems-level knowledge and biological validation to develop novel therapeutic and diagnostic approaches to extend survival in myeloma and other hematologic malignancies.

NEWS & NOTEWORTHY

9/25/17 — We are happy to welcome Catherine Tan for her BMS rotation!

8/17/17 — We are happy to welcome our new Junior Specialist, Nora Lam!

7/7/17 — We wish Christine luck in starting graduate school at Johns Hopkins!

7/1/17 — We thank the UCSF Program in Breakthrough Biomedical Research for supporting our collaborative project with the Blelloch lab!

6/28/17 — Congrats to Hector, his paper on measuring protein synthesis by pulsed-SILAC proteomics and ribosome profiling, in collaboration with Yun Song's lab, was published in Cell Systems! Thanks to Neil Wiita for excellent cover art.
CellSystemsCover

6/18/17 — We are happy to welcome our new Junior Specialist in the MMTI Lab, Ian Ferguson!

6/10/17 — We wish Shubhang luck in starting medical school at the Mayo Clinic Arizona!

5/28/17 — Check out Christine's manuscript on drug repurposing in myeloma posted at BioRxiv!

5/17/17 — We are happy to welcome our new Associate Specialist in the MMTI Lab, Priya Choudhry!

4/10/17 — We are happy to welcome our new Research Associate in the MMTI Lab, Margarette Mariano!

3/6/17 — We are happy to welcome our new Junior Specialist in the MMTI Lab, Shubhang Bhatt!

2/20/17 — We thank the Gabrielle's Angels Foundation for Cancer Research for supporting our group with a Research Grant!

1/6/17 — Congrats to Hui on her new Scientist position at GenEdit! We wish her luck in making CRISPR therapeutics a reality.

1/3/17 — We are happy to welcome our new Post-doc, Neha Paranjape!

12/15/16 — We thank the Multiple Myeloma Research Foundation for supporting our group with a Translational Network Research Award!

11/15/16 — Check out Hector's new paper, a collaboration with Yun Song's group, posted at BioRxiv!

10/4/16 — We thank the NIH Common Fund for the supporting our group with the NIH Director's New Innovator Award!

8/15/16 — We are happy to welcome our new Junior Specialist, Tony Lin!

7/1/16 — We thank the Doris Duke Charitable Foundation for supporting our group with the Clinical Scientist Development Award!

2/16/16 — We are happy to welcome our new Post-Doc, Sami Tuomivaara!

10/3/15 — We are happy to welcome our new Post-Doc in the MMTI Lab, Hui Liu!

9/3/15 — We are happy to welcome our new Junior Specialist in the MMTI Lab, Christine Lam!

6/10/15 — We are excited to be part of the UCSF MMTI team, as the Stephen and Nancy Grand Multiple Myeloma Translational Initiative Laboratory joins our group.

2/4/15 — We are happy to welcome our new Post-Doc, Matt Nix!

1/27/15 — We thank the Damon Runyon Cancer Research Foundation for supporting our group with the Dale F. Frey Breakthrough Award.