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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Test code CHOL
Performed by? Chemistry
Sendout? no
Price range $
In House Availability 7 days
Principle Enzymatic

Hydrolysis of the cholesterol esters to cholesterol is catalyzed by cholesterol ester hydrolase and the cholesterol is then oxidized in the presence of cholesterol oxidase to form cholestenone and hydrogen peroxide. A colored complex is formed from hydrogen peroxide, 4-aminoantipyrine and phenol under the catalytic influence of peroxidase.
Interpretation 1. Hypercholesterolemia: uncontrolled diabetes; nephrotic syndrome; obstructive jaundice; untreated myxedema; hereditary lipoprotein abnormalities with premature arteriosclerosis.

2. Hypocholesterolemia: hyperthyroidism; pernicious anemia; hepatic disease. Severe systemic infections result in a hypermetabolic state with hypocholesterolemia, malnutrition, and/or malabsorption. Tangiers disease associated with deficiency of alpha-lipoprotein.
Special instructions Fasting required for triglyceride for calculation of LDL-Cholesterol.
Normal range
Desirable < 200 mg/dL < 5.17 mmol/L
Borderline 200-239 mg/dL 5.17-6.18 mmol/L
High Risk ≥ 240 mg/dL ≥ 6.21 mmol/L

Synonyms HDL, Cholesterol;Lipid Panel (CHOL, HDL, LDL, TRIG);
Stability Total cholesterol, 3 days at 4°C; 3 months at -20°C.
Interferences Certain drugs can interfere with the measurement of cholesterol in vivo and in vitro. See Young, D.S (reference #6, below).
References 1. Allain, C.C., et al.: Enzymatic determination of total serum cholesterol. Clin Chem, 20:470, 1974.

2. Bayer(TM) Clinical Methods Cholesterol (21) B01-4124-01 Advia 1650 Operator's Guide. PN 073-5001-09. Last accessed June 2003. Siemens Medical Solutions Diagnostics, Malvern, PA.

3. Fredrickson, D.S., Levy, R.I., Lees, R.S.: Fat transport in lipoproteins - an integrated approach to mechanisms and disorders. NEJM, 276:148-156, 1967.

4. Naito, H.K., Clin Chem, 34/2:444-9, 1988.

5. National Cholesterol Education Panel, Lipid Metabolism Branch, Division Heart, Lung, and Blood Institute. US Dept of Health and Human Services, Public Health Services, National Institutes of Health Bethesda, MD, JAMA 2001:285:2486-97.

6. Young, DS. Effects of Drugs on Clinical Laboratory Tests. Ed. 4. Washington D.C.: AACC Press; 1995.
CPT coding 82465
Last Updated 10/19/2012 1:45:46 PM
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