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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Approval req'd? No
Available Stat? No
Test code STX
Performed by? Microbiology (Bacteriology)
Sendout? No
Price range $$
In House Availability Specimen may be sent to the Laboratory 24 hours/7 days. Test is performed once per day, on the day shift, 7 days a week.
Principle This test is for the simultaneous qualitative detection and differentiation of Shiga toxin 1 and Shiga toxin 2 produced by toxigenic strains of Escherichia coli .
Method Rapid membrane enzyme immunoassay
Container type Para-Pak C&S vial (Cary-Blair transport medium)
Amount to Collect 5 mL
Collection Instructions Collect stool in Para-Pak C&S vial, available through Lab Support Services (415-206-8199). Multi-language directions in the collection kit describe proper collection.

If stool is submitted in sterile, screw cap container, it must be received in the Microbiology Laboratory within 2 hours of collection.
Sample type Stool
Min. Volume 5 mL
Normal range Negative for Shiga Toxin 1 and Shiga Toxin 2
Synonyms E. coli O157; Hemolytic Uremic Syndrome (HUS);
Stability Unpreserved: 2 hours.
Preserved in Para-Pak C&S vial and refrigerated 2° - 8°C: 3 days.
Causes for rejection 1. Unpreserved stool (in sterile cup) received > 2 hours after collection.
2. Stool in Para-Pak C&S vial received > 72 hours after collection.
3. Stool in any formalin-based or alcohol-based fixatives.
4. Stool collected on swabs or culturettes; i.e., rectal swabs.
5. Leaking specimens.
6. More than one specimen collected within 24 hours.
7. Specimens submitted within 4 days of a prior POSITIVE result.
8. Specimens submitted less than or equal to 24 hours of a prior NEGATIVE result.
Turn around times 2 days
Reflex? Culture for E. coli O157 and Shiga Toxin Assay are automatically performed on stool submitted for bacterical culture. Each test is billed separately.
References Alere Shiga Toxin Quik Chek product insert, 10/2012. EMS#91-625-01.
CPT coding 87427 x2
Last Updated 1/26/2016 8:45:31 AM
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