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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Test code MC
Performed by? Microbiology (Bacteriology)
Sendout? no
Price range $$$
In House Availability 7 days
Interpretation H. ducreyi is the causative agent of chancroid (soft chancre), a sexually transmitted disease. Chancroid is characterized by one or more deep, tender, friable genital ulcers with ragged, undermined edges and a purulent base. The incubation period is usually 3-5 days, often shorter than syphilis. About 50% of patients have painful or tender adenopathy.
Collection Instructions Use a preservative-free saline or broth-moistened swab to collect purulent material from the ulcer base. Inoculate directly onto 1/3 of the H. ducreyi (Nairobi) agar plate and chocolate agar plate. Alternately, send a swab in transport media or send pus or biopsy material in sterile tube.
Special instructions Call Microbiology (206-8576) to obtain H. ducreyi media (Nairobi agar) and chocolate plate immediately before culture is taken. Also, Dermatology (Resident pager 443-9274) would like to be notified when a patient comes in with suspected chancroid.
Synonyms Chancroid, Haemophilus Ducreyi Culture;
Stability H. ducreyi is a fastidious organism, susceptible to heat and drying. For optimal recovery, bring specimen to Microbiology Lab (2M33) immediately after collection.
References 1. Dangor Y, Ballard RC, Miller SD, Koornhof HJ. Treatment of Chancroid, Antimicrob. Agents Chemother. 34: 1308-1311.

2. Kilian M. Chapter 41 - Haemophilus in Murray, PR et al., Manual of Clinical Microbiology, 9th Ed., ASM Press, Washington, DC, 2007.
Last Updated 8/19/2009 1:18:51 PM
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