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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Approval req'd? no
Available Stat? No
Test code UDIP: Routine (reagent dipstick) urinalysis with reflex to urine microscopy if indicated.

DM: Routine (reagent dipstick) urinalysis and urine microscopy.

UMIC: Urine microscopy.

Performed by? Hematology
Sendout? no
Price range $
In House Availability 24 hours/ 7 days
Principle The primary method for urinalysis is the Aution Hybrid AU-4050 Automated Urinalysis System, which includes a chemistry test strip module and a flow cytometry module. The Iris Diagnostics iChem(R) 100 is also available as a back-up method for automated urine chemistry analysis.

The automated reader (AU-4050) is used with a reagent strip (dipstick) methodology to make semi-quantitative measurements of chemical analytes (pH, glucose, ketones, blood, protein, and bilirubin) and to screen for bacterial infection by detection of leukocyte esterase and nitrite. Specific gravity is measured by a refractometer for this method. Whenever the primary automated analyzer cannot be used the backup analyzer (iChem(R) 100) is used and manual urine microscopy is performed, if necessary. The backup automated urine analyzer measures specific gravity by the ion concentration method.

Urine microscopy is performed at the request of the ordering provider. Microscopic examination of urine sediment is also performed by reflex whenever protein, blood, leukocyte esterase or nitrite is positive by the reagent strip method. Positive tests for leukocyte esterase and nitrite may provide presumptive evidence of infection but should be confirmed by urine culture. Quantitative analysis of urine analytes may be requested through urine chemistry as a separate test.

Container type urine cup
Amount to Collect 20 ml (minimum 0.5 ml for dip stick analysis, 3 ml for microscopic examination
Collection Instructions Preferably clean catch, early morning specimen.
Normal range
leukocyte esterase = negative
nitrite = negative
specific gravity = 1.003 - 1.030
pH = 5.0 - 7.5
protein = negative
glucose = negative
ketone = negative
bilirubin = negative
blood = negative
WBC = 0 - 2/HPF
RBC = 0 - 3/HPF
hyaline cast = none to few (0 - 3/HPF)

Stability Room temperature: 2 hours; Refrigerated: 24 hours. If delivery time to the lab is expected to exceed 2 hours the specimen should be refrigerated immediately after collection. If the specimen cannot be delivered within 2 hours (room temperature) or 24 hours (refrigerated), the patient should be sent to the main clinical laboratory at SFGH for collection. Specimens exceeding the listed stability times may show degradation of chemical analytes and formed elements in urine sediment, which may result in false negative results.
Additional information INDICATIONS: Routine initial examination of patient; numerous other conditions.

PEDIATRIC PATIENTS: Routine urinalysis does not include testing for reducing substances, such as the sugars galactose, lactose, fructose, and maltose. If clinically indicated, a screening test for reducing substances may be ordered as a send out test. Testing for congenital galactosemia is included in newborn screening programs in all 50 states in the U.S.
References Fuller, CE et al: Basic Examination of Urine. In: Henry, JB: clinical Diagnosis and Management by Laboratory Methods, 20th ed. Philadelphia, WB Saunders Co, 2001, pp 367-402.
CPT coding 81003, 81001, 81002, 81000
Last Updated 4/9/2018 9:09:24 AM
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