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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Test code URIC
Performed by? Chemistry
Sendout? no
Price range $
In House Availability 7 days
Principle Uricase catalyzes oxidation of uric acid to allantoin and H2O2. H2O2 reacts with 4-aminophenazone and TOOS [N-ethyl-N-(2-hydroxy-3-sulfopropal)3-methylaniline] to produce a colored compound in the presence of peroxidase. The absorbance of the colored complex is proportional to the concentration of uric acid in the sample.
Interpretation Uric acid fluctuates widely in the same individual from day to day, with seasonal variations, and is varied by diet (increased by meat, liver, legumes, mushrooms, and spinach); increased by fasting and emotional stress.

1. Increased with gout and 25% of relatives of patients with gout; renal failure; with diuretics; following antimetabolite and radiation therapy of leukemia, lymphoma, multiple myeloma or polycythemia vera; in psoriasis and alcoholism; in patients with arteriosclerosis and hypertension. Serum uric acid is increased in more than 80% of patients with elevated triglycerides. Increased in various populations (Blackfoot and Pima Indians, Filipinos, etc.).

2. Decreased in administration of high doses of salicylates, probenecid, cortisone, allopurinol, coumarin; in Wilson's disease, Fanconi's syndrome, acromegaly, celiac disease, xanthinuria.
Container type gel tube (gold or green top)
Amount to Collect 2 mL
Sample type Blood
Normal range Serum

Age mg/dL mcmol/L
1-16 yrs 2.0-6.0 119-357
> 16 yrs
Female 2.5-7.5 149-446
Male 3.5-8.5 208-506
Stability Stable at 2-8°C for 3 days; frozen for 6 months.
Interferences Many drugs, including thiazides, increase uric acid concentrations. Other substances (ascorbic acid, gentisic acid, alpha-methyl dopa, xanthine, sodium citrate, acetaminophen, hydralazine, and allopurinol) decrease uric acid.
References 1. Bayer(TM) Clinical Methods Uric Acid (47) B01-4131-01 Advia 1650 Operator's Guide. PN 073-5001-09. Last accessed June 2003. Siemens Medical Solutions Diagnostics, Malvern, PA.

2. Tietz NW (ed). Fundamentals of Clinical Chemistry, ed. 5. Philadelphia: WB Saunders, 422-426, 2001.

3. Young, DS. Effects of Drugs on Clinical LaboratoryTests. Ed. 4. Washington D.C.: AACC Press; 1995.
CPT coding 84550
Last Updated 8/19/2009 1:32:57 PM
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