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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Available Stat? No
Test code TRSF
Performed by? Chemistry
Sendout? no
Price range $$$
In House Availability Monday - Friday
Principle The Transferrin method is a polyethylene glycol (PEG) enhanced immunoturbidimetric assay. Sample containing human transferring is suitable diluted and then reacted with specific antiserum to form a precipitate that can be measured turbidimetrically at 596 nm. By constructing a standard curve from the absorbance of standards, transferring concentration of sample can be determined.
Interpretation Transferrin
1. Increased: iron deficiency anemia, late pregnancy, infancy, ingestion of oral contraceptives, hepatitis, chronic blood loss.

2. Decreased: nephrosis via loss of transferrin through the glomerulus, or decreased in malignancy, starvation, and in chronic inflammation due to increased catabolism. Also decreased with hemochromatosis, cirrhosis of the liver, and thalassemia.

1. Increased: with decreased transferrin (nephrosis, hepatitis, kwashiorkor); increased with thalassemia, lead poisoning, pyridoxine deficiency, hemochromatosis, and hemosiderosis.

2. Decreased: iron deficiency anemia, anemias of infection and chronic disease (uremia, RA, neoplasms, etc.).
Container type gold top gel tube
Amount to Collect 2 mL
Sample type Blood
Special instructions SPECIAL INSTRUCTIONS: Slight to moderately hemolyzed specimens will be assayed, but not grossly hemolyzed specimens. Fasting specimen is preferred. In order to receive % saturation, both an iron and Transferrin must be ordered on the same specimen.
Normal range
Iron, serum Male: 65 - 175 mcg/dL
Female: 50 - 170 mcg/dL

Transferrin 180 - 335 mg/dL (1.80 - 3.35 g/L) IFCC
% Saturation 16 - 60%

TIBC = Transferrin x 1.25

%Saturation = [Serum Iron] / [TIBC] = [Iron] / [Transferrin x 1.25]

Synonyms Iron-Binding Capacity (TIBC);TIBC;
Stability 72 hours at 2-8°C.
Interferences Hemolysis releases free hemoglobin to raise serum iron.
References 1. Henry J: Clinical Diagnosis and Management by Laboratory Methods. 20th ed., WB Saunders, 2001, pp 254-258.

2. Beilby, J., et al., "Transferrin Index: An Alternative Method for Calculating the Iron Saturation of Transferrin," Clin. Chem. 38(10): 2078-2081 (1992).

3. Tietz, N.W. Textbook of Clinical Chemistry and Molecular Diagnosis, ed. 4. St. Louis, MO: Elsevier Saunders, 562-563; 2006.

4. Gambino, R., et al., "The Relation Between Chemically Measured Total Iron-Binding Capacity Concentrations and Immunologically Measured Transferrin Concentrations in human Serum," Clin. Chem. 43(12): 2408-2412 (1997).

5. Beckman Immage Chemistry Information Manual 962248 December 1996. Beckman Coulter, Inc. Fullerton, CA.
CPT coding 84466
Last Updated 10/23/2012 4:55:54 PM
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