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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Test code RUBG
Performed by? Chemistry
Sendout? no
Price range $
In House Availability Monday - Friday
Principle The Rubella IgG assay is an IgG antibody capture microparticle direct chemiluminometric immunoassay for the quantitative detection of IgG antibodies to rubella virus in human serum as an aid in the assessment of immune status to rubella in individuals, including women of childbearing age.
Interpretation Rubella is a member of the togaviridae family. Primary infections are generally mild, with symptoms such as a mild rash and low-grade fever. In contrast, primary infections during pregnancy can pass transplacentally to the fetus and can lead to fetal death or congenital rubella syndrome (CRS). A primary infection induces an IgM and an IgG response. Within 4 to 6 months, IgM levels become undetectable or very low. IgG decreases to low levels, but lasts indefinitely and confers lifelong immunity. A secondary infection exhibits a rising IgG antibody without significant levels of IgM. An antibody level of 10 IU/mL is thought to be protective.

For documentation of current acute infection, the San Francisco Department of Public Health (SFDPH) will arrange testing (5 mL clotted blood in gel tube) on paired acute and convalescent specimens drawn 2-3 weeks apart. The SFGH Clinical Microbiology Laboratory will hold acute serum (frozen) until the convalescent specimen is obtained. Acute and convalescent specimens will be sent together to the SFDPH.
Container type gold top gel tube; red non-gel tube acceptable
Amount to Collect 2 mL
Sample type Clotted Blood
Normal range Values ≥ 10.0 IU/mL are considered reactive, based on the WHO International Standard for Anti-Rubella Serum as an indicator of immune status and a breakpoint to detect most seropositive persons.
Stability 7 days at 4°C
Additional information REQUISITION: Blood/Serum - Main Laboratory
References 1. Armstrong AS, Safford JW, Holbert DN, Mushahra IK. Congenital diseases of microbiological origin. In: The Immunoassay Handbook: Applications. Edited by David Wood. New York: Stockton Press, 1994:499-501.

2. National Committee for Clinical Library Standards. Detection and quantitation of rubella IgG antibody: evaluation and performance criteria for multiple component test products, specimen handling, and use of test products in the clinical laboratory; approved guideline. NCCLS Document I/LA6-A. Wayne(PA): NCCLS; 1997 Oct. 24p.

3. Mandell GL, Bennett JE and Dolin R., Eds. Principles and Practice of Infectious Diseases, 6th ed., Churchill Livingstone, Inc., New York, 2005.

4. Detrick B, Hamilton RG, Folds, JD, Eds. Manual of Molecular and Clinical Laboratory Immunology, 7th Edition, ASM., Washington, DC, 2006.
CPT coding 86762
Last Updated 10/23/2012 4:54:22 PM
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