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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Test code PCE
Performed by? Microbiology (Mycology)
Sendout? no
Price range $$$
In House Availability Monday - Friday, 8:00 am - 2:00 pm
Method Pneumocystis is identified morphologically by microscopic examination of Giemsa or Diff-Quik (modified Giemsa) stained preparations.
Container type sterile, screw-capped container
Sample type bronchoalveolar lavage, transbronchial lung biopsy tissue
Special instructions Examinations for Pneumocystis are arranged through the Chest Service Fellow (pager 443-6214). Bronchoalveolar lavage (large volume) specimens obtained during fiber-optic bronchoscopy provide the greatest yield of positive specimens. Transbronchial or open lung biopsy is infrequently necessary for diagnosis of Pneumocystis infection although other infectious agents (e.g., fungus) may be found in this specimen.

Please alert the Lab when Cryptococcus, Histoplasma, mycobacteria, or Toxoplasma should be searched for in these specimens (bronchoalveolar lavage or tissue touch preparations).

Proper handling of transbronchial lung biopsy tissue is essential for obtaining good touch preparations. Biopsies should be placed on a sterile non-bacteriostatic saline moistened gauze or Telfa in a sterile screw-cap container and brought immediately to the Microbiology Laboratory. Biopsies must not be transported either dry or immersed in saline.

Always call the Microbiology Resident (206-5699, or pager 415-443-1438) prior to starting an open lung biopsy for touch preparations and culture.
Normal range negative
Synonyms PCP;
Stability Deliver immediately to the Microbiology Laboratory (2M33).
Causes for rejection tissue submitted floating in saline
References 1. Murray PR, et al. Manual of Clinical Microbiology, 9th Ed., ASM Press, Washington, DC. 2007.

2. Ng VL, Gartner I, Weymouth LA, et al: The Use of Mucolysed Induced Sputum for the Identification of Pulmonary Pathogens Associated with Human Immunodeficiency Virus Infection. Arch Pathol Lab Med. Vol 113 (May) 1989, p 488-493.

3. Ng VL, Yajko DM, McPhaul LW, et al: Evaluation of an Indirect Fluorescent-Antibody Stain for Detection of Pneumocystis carinii in Respiratory Specimens. J Clin Microbiol. 1990 28: 975 – 979.
CPT coding 88104
Last Updated 4/30/2011 10:47:50 AM
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