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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Test code FCB
Performed by? Microbiology (Mycology)
Sendout? no
Price range $$
In House Availability 7 days
Principle The Microbiology Laboratory uses the bioMérieux BacT/ALERT continuous monitoring system FAN aerobic (green) bottle to culture for fungi. After incubation for 5 days, an aliquot is removed from the FAN aerobic bottle, centrifuged, and the sediment inoculated to fungal culture media. The cultures are incubated for 4 weeks. A technologist will report by telephone any blood culture that becomes positive. Negative blood cultures are reported as no fungus recovered after 4 weeks incubation.
Container type FAN aerobic blood culture bottle, (green cap), available from Laboratory Support Services (206-8199). The FAN aerobic blood culture bottles are used when both fungal and bacterial cultures are desired. If both bacterial and fungal cultures are desired, both must be checked-off on the requisition. Fungal cultures are given additional processing and incubation time after the routine bacterial culture is completed.
Collection Instructions COLLECTION AND HANDLING: The preparation of the venipuncture site is extremely important in the collection of a blood culture. The site should be prepared meticulously by scrubbing with Chloraprep applicator sponge, and care should be taken to avoid subsequent contamination of the area by finger palpation. Sterilize the exposed rubber diaphragms of the blood culture bottles with sterile alcohol. Do not use any other sterilizing agent. Withdraw blood using Safety-Lok needle and direct draw adapter (available in blood culture bottle kits). Add 5 to 10 mL (10 mL recommended) of blood into each bottle, being careful not to inject any air bubbles into the anaerobic bottle (purple cap). DO NOT add more than 10 mL of blood to the bottle. Record the patient's name and medical record number on a large removable label and place it over the bottle labels. Complete one requisition per 2 bottle set.

If you are drawing a blood culture for Fungal only, just the FAN (green cap) bottle of the set needs to be inoculated as pathogenic fungi do not grow anaerobically. Return the entire set, with the requisition to Microbiology. If you suspect Actinomyces or Nocardia, send both anaerobic and aerobic bottles.

Sample type Blood
Synonyms Blood Culture;Fungus Culture, Blood;Histoplasma Culture;
Stability Leave blood culture bottle at room temperature until delivery to the Laboratory.
Additional information Blood Culture for Histoplasma
Histoplasma capsulatum can be recovered from routine fungal blood cultures. Histoplasma organisms may be found intracellular in phagocytic cells. They may be detected by microscopic exam of the regular peripheral smears.
References 1. bioMérieux BacT/ALERT 3D Technical Information. bioMérieux Inc., Durham, NC. 1999.

2. Larone DH. Medically Important Fungi, 4th Ed., ASM Press, Washington, DC. 2003.

3. Murray PR, et al. Manual of Clinical Microbiology, 9th Ed., ASM Press, Washington, DC, 2007.
CPT coding 87103
Last Updated 8/19/2009 1:32:19 PM
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