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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources


Item Value
Available Stat? No
Test code AFNS
Performed by? Microbiology (AFB)
Sendout? no
Price range $$$
In House Availability 7 days
Principle Specimen is decontaminated and concentrated in order to prevent overgrowth by other organisms. The concentrated sediment is inoculated into AFB culture media and incubated for up to 8 weeks. An acid-fast smear is not performed on the sediment as it may contain non-pathogenic mycobacteria which would appear acid-fast on a smear. Negative results are finalized after 8 weeks. Positive results are reported as soon as AFB are recovered.
Patient Preparation The patient must not be allowed to brush his/her teeth with toothpaste prior to this collection. Specimens that contain toothpaste or food particles are not satisfactory.
Container type sterile, screw-capped container
Collection Instructions Collect a fasting early-morning specimen in order to obtain sputum swallowed during sleep.
Sample type Gastric aspirate
Special instructions The specimen must be brought immediately to the laboratory for neutralization of acidity to prevent destruction of mycobacteria.
Stability Deliver to Microbiology Laboratory (2M33) immediately.
Additional information Induced sputum or bronchoscopic aspirate is always preferable to gastric aspirates.
References Kent, PT and GP Kubica. 1985. Public Health Mycobacteria: A Guide for the Level III Laboratory. U.S. Department of Health and Human Services. CDC. Atlanta GA.
Last Updated 8/19/2009 1:32:06 PM
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