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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources


Item Value
Available Stat? No
Test code AFBB
Performed by? Microbiology (AFB)
Sendout? no
Price range $$
In House Availability 7 days
Principle Blood is inoculated into AFB culture media and incubated for up to 6 weeks. Negative blood cultures are finalized after 6 weeks. Positive cultures are reported as soon as AFB are recovered.
Container type Yellow top SPS (sodium polyanetholesulfate) tube
Amount to Collect 5 mL (3mL minimum)
Collection Instructions COLLECTION AND HANDLING: The preparation of the venipuncture is extremely important in the collection of a blood culture. The site should be prepared meticulously by scrubbing with Chloraprep applicator sponge. Allow site to air dry completely; care should be taken to avoid subsequent contamination of the area by finger palpation. Sterilize the top of the yellow top SPS tube with alcohol. Do not use any other sterilizing agent. Withdraw blood using standard blood collection needle and holder or a Safety- Lok needle and direct draw adapter ("butterfly," available in blood culture kits). Draw 5 mL of blood into tube. Immediately after collection, gently invert tube 8 times to prevent clotting. Place label with patient's name and medical record number on the tube. Note time and date of collection on requisition.
Sample type Whole blood
Min. Volume 3 mL
Special instructions Gel tubes are unacceptable. Bright green top (sodium heparin) tubes are unacceptable. Standard aerobic, anaerobic, or FAN blood culture bottles, are unacceptable.

SPS tubes are only available as glass tubes; therefore specimen must be delivered by messenger. DO NOT send SPS tubes to the Laboratory through the pneumatic tube system.
Synonyms Blood Culture;MAC Culture;AFB Blood Culture
Stability Specimen must be maintained at room temperature and received by the Microbiology Laboratory within 24 hours of collection.
Additional information In AIDS patients, blood may yield Mycobacterium avium complex (MAC), and occasionally other species of mycobacteria.
References Kent, PT and GP Kubica. 1985. Public Health Mycobacteria: A Guide for the Level III Laboratory. U.S. Department of Health and Human Services. CDC. Atlanta GA.
Last Updated 8/12/2015 1:47:13 PM
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