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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Test code LD
Performed by? Chemistry
Sendout? no
Price range $
In House Availability 7 days
Principle Lactate dehydrogenase catalyzes the conversion of lactate and NAD to pyruvate and NADH. The rate of NADH production is proportional to LD activity.
Interpretation LD occurs in all organs, but elevation occurs in the serum only after organ damage.

1. Greatest increases (2-40x) occur with megaloblastic anemia, extensive carcinomatosis, and with severe shock, rhabdomyoloysis, and anoxia.

2. Moderate increases (2-4x) occur with MI, but also pulmonary infarction, granulocytic anemia, hemolytic anemia, mononucleosis, muscular dystrophy. Following MI (24 hr), increased LD occurs and may persist 10-14 days.

3. Mild increases: hepatitis, delirium tremens (skeletal muscle origin), and muscular dystrophy. Isoenzyme electrophoresis can determine the possible organ source of LD.

4. Decrease: Could be due to storage, certain drugs, or inhibitors.
Container type gel tube (gold or green top)
Amount to Collect 2 mL
Sample type Blood
Special instructions Hemolyzed serum will not be assayed.
Normal range
Age U/L Range
0 - 14 days 180-430
2 wks - 2 yrs 110-295
2 - 12 yrs 100-190
> 12 yrs 110-210

Synonyms LD;
Stability Serum stable 2-3 days at 2-8°C. Do not freeze. Certain LDH isoenzymes (LD-4 and LD-5) are more sensitive to cold. Specimens may have variable decreases in LD activity when stored at 2-8°C, depending on their isoenzyme composition.
References 1. Wacker, W.E.C., Ulmer, D.D., Vallee, B.L.: Metalloenzymes and myocardial infarction, malic and lactic dehydrogenase activities and zinc concentrations in serum. NEJM 255:449, 1956.

2. Cheng, M.S., Lipsy, A.I., Blanco, V. et al: Microchemical Analysis for 13 Constituents of Plasma from Healthy Children. Clin Chem, 25:5, 1979.

3. Bayer(TM) Clinical Methods LDH LP (71) B01-4142-01 Advia 1650 Operator's Guide. PN 073-5001-09. Last accessed June 2003. Siemens Medical Solutions Diagnostics, Malvern, PA.
CPT coding 83615
Last Updated 11/11/2010 4:09:39 PM
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