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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Performed by? Chemistry
Sendout? no
Principle Protein electrophoresis followed by addition of monovalent IgG, IgA, IgM, kappa or lambda antiserum results in immunoprecipitation of the abnormal bands. Abnormal protein bands are immobilized with a fixative.
Interpretation Characterization and identification of monoclonal and abnormal protein bands in urine or serum.
Container type gold top gel tube
Amount to Collect 2 mL blood, or 10 mL urine
Collection Instructions Hemolyzed specimens will not be assayed.
Sample type Blood or urine
Special instructions Immunofixation electrophoresis is performed only when protein electrophoresis is abnormal or under special request. For further details contact the Laboratory Medicine Resident in Clinical Chemistry (x65527), or pager (415) 443-2311.
Stability 7 days at 2-8°C.
References 1. Keren D.F., "High Resolution Electrophoresis and Immunofixation Techniques and Interpretations," Butterworth-Heinemann, Woburn, MA, USA, 1994, p.397.

2. Le Carrer, D. and Boucraut, J.: Urine Protein Electrophoresis Immunofixation, Illustrated Interpretations, Sebia Labs, Hatier, Paris (1999).

3. Le Carrer, Didier; "Serum Protein Electrophoresis and Immunofixation, Illustrated Interpretations," SEBIA Laboratories, 1994, Hatier-Paris.

4. Ritchie, R.F., Smith, R: Clin Chem, 22, p. 497 (1976).

5. Sebia Hydragel 2IF Acid Violet Reagent Insert Ref. 4152, Sebia, Inc., Norcross, GA (Dec., 2000).

6. Sun, T. Physiology of Immunoglobins: Diagnostic and Clinical Aspects. Liss, N.Y. (1982) pp. 97-115.
Last Updated 4/12/2012 6:06 PM
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