HEMOGLOBINOPATHY EVALUATION
| Item | Value | ||||
|---|---|---|---|---|---|
| Available Stat? | No | ||||
| Test code | HGBV | ||||
| Performed by? | Chemistry | ||||
| Sendout? | no | ||||
| Price range | $$$ | ||||
| In House Availability | Once a week, usually on Wednesdays. Requests for urgent testing between routine run dates will be considered individually, based upon medical necessity. For approval, call the Clinical Laboratory Information Section (Inquiry) at 415-206-8590, and request their assistance to contact the Lab Medicine Resident. | ||||
| Principle | Hemoglobins are separated and quantitated by an ion-exchange high performance liquid chromatography method (IE-HPLC, VARIANT II system, Bio-Rad). The method is based on electrical charge differences between hemoglobin molecules and the stationary phase in a chromatography column, resulting in distinct retention times for hemoglobin variants as they pass through the column. | ||||
| Interpretation | QUANTITATIVE RESULTS, INCLUDING HEMOGLOBIN A2, F: * Hemoglobins A2, F, and S and C, if present, will be quantitated and reported. If MCV is below the reference range (< 80 fL), ZNPP (zinc protoporphyrin) will automatically be performed to evaluate for iron deficiency; the results will be reported. * For quantitative A2, F order "Hemoglobinopathy Evaluation" INTERPRETATION: For interpretation, consult the Laboratory Medicine Resident in Hematology (x65689; pager: 415-443-0179; pager, after hours and on weekends: 415-443-6969). |
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| Container type | EDTA (lavender top) tube | ||||
| Amount to Collect | 5 mL | ||||
| Collection Instructions | Mix tube well to avoid clotting. | ||||
| Sample type | Whole blood | ||||
| Normal range |
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| Synonyms | Hemoglobin A2;Hemoglobin Electrophoresis, Qualitative;Hemoglobin F; | ||||
| Additional information | Include clinical history and order CBC. Both are required for interpretation. If no CBC is ordered, the lab will add this test. CONFIRMATION OF ABNORMAL HEMOGLOBINS: If there is no prior record of an abnormal hemoglobin, the specimen will be sent out to a sendout lab for confirmation. Other confirmatory tests which may be performed include sickle cell solubility test and hemoglobin H preparation. |
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| References | 1. Bain, BJ: Haemoglobinopathy Diagnosis. Blackwell Science Ltd, 2001. 2. Waters et al.: An evaluation of the Bio-Rad Variant Haemoglobin Testing System for the detection of haemoglobinopathies. Clin Lab Haematol 20:31-40, 1998. 3. Instruction Manual for the Bio-Rad VariantªII ?-thalassemia Short Program. 702:037-2. Oct 2002. BioRad Laboratories, Hercules, CA. |
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| CPT coding | 83021 |
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| Last Updated | 10/18/2017 11:37:49 AM |