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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Approval req'd? yes, Restricted Test
Available Stat? No
Test code HPYL
Performed by? Reference Laboratory - ARUP web page for Helicobacter pylori Antigen, by EIA .
Sendout? yes
Price range $$
Principle This test is a qualitative enzyme immunoassay that detects H. pylori specific antigens present in stool of infected individuals. This assay indicates active infection with (antigen), not just exposure to (antibody) H. pylori.
Interpretation Though a causative relationship has not been fully established, there is a strong correlation between the presence of H. pylori in stool and histologically confirmed gastritis, peptic ulcer disease, and gastric carcinoma.

A positive test result indicates the presence of H. pylori specific antigens and is intended to aid in the diagnosis of H. pylori infection. A negative result indicates the absence of H. pylori antigens, or the level of antigens is below that which can be detected by the assay.
Container type sterile, screw-capped container without preservatives or diluents
Collection Instructions Do not submit stool specimens for Helicobacter pylori antigen in C&S vials, SAF vials, or on swabs. Deliver to Microbiology lab (2M33) as soon as possible after collection or hold at 4°C until delivery.
Sample type fresh stool less than 24 hours old, at refrigerated temperature. (Note: Specimen is stable for only 2 hours at room temperature.)
Min. Volume 1 mL, or piece the size of a large marble (1 gram)
Normal range negative
Interferences LIMITATIONS: Antimicrobials, proton pump inhibitors, and bismuth preparations are known to suppress H. pylori , and ingestion of these prior to H. pylori antigen testing may give a false negative result. Wait 2 weeks after discontinuing treatment before collecting specimen for H. pylori antigen testing.
Causes for rejection Stool greater than 24 hours old
Stool in ParaPak for C & S (Cary Blair transport medium)
Stool in SAF for Ova & Parasite exam
Stool in any other media or preservatives
Leaking specimens
Swab specimens
Gastric or tissue specimens
Additional information REQUISITION: Microbiology
CPT coding 87338
Last Updated 6/29/2016 3:51:17 PM
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