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Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

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Item Value
Available Stat? No
Test code GIA
Performed by? Microbiology (Parasitology)
Sendout? no
Price range $$$
In House Availability Mon. - Fri., day shift
Principle This test is a solid phase immunoassay for the detection of Giardia-Specific Antigen 65 (GSA 65) in aqueous extracts of human fecal specimens. GSA 65 is a 65,000 kd molecular weight glycoprotein that is produced in abundant quantities by the Giardia protozoa as they multiply within the host intestinal tract. It is not degraded by bacterial and host enzymes in the intestine. Secretion of GSA 65 is not dependent on excretion of cysts or trophozoites in the stool and is present only when giardial infection is present.
Container type Parasitology SAF Fixative Collection vial. Para-Pak C&S vial is also acceptable.
Collection Instructions Follow instructions which accompany each SAF Fixative Collection Kit, available from Lab Support Services 206-8199. Only 1 specimen per 7 day time period will be accepted and tested for each patient.
Sample type Stool
Normal range Negative.
Synonyms Parasites, Giardia Antigen Test;
Stability Specimens placed in SAF are stable at refrigerated or room temperature for 7 days. Specimens placed in Para-Pak C&S vial are stable at refrigerated temperature for 72 hours.
References 1. Prospect(R) Giardia EZ Microplate Assay Product Insert, Alexon-Trend, Inc., Ramsey, MN. 2003.

2. Rosoff JD, et al., Stool diagnosis of Giardiosis using a commercially available enzyme immunoassay to detect Giardia-Specific Antigen 65 (GSA 65). Journal of Clinical Microbiology. 27: 1997-2002, 1989.
CPT coding 87329
Last Updated 11/20/2010 8:43:19 AM
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