Sensitivity

The sensitivity of the AxSYM Cannabinoids assay was caluclated to be 13.00 ng/mL. This sensitivity is defined as the lowest measurable concentration which can be distinguished from zero with at least 95% confidence.

Specificity

Cross-reactivity was tested for the commonly used cannabinoids and metabolites. The following compounds cross-react above the sensitivity (13.00 ng/mL) of the AxSYM Cannabinoids assay:

Test Compound	Concentration Tested (ng/mL)
11-Nor-delta-8-THC-9-carboxylic Acid	25
11-OH-delta-9-THC	25
Cannabinol	25

Cross-reactivity was tested with compounds that have similar chemical structure or are used concurrently. The following compound cross-reacts above the sensitivity (13.00 ng/mL) of the AxSYM Cannabinoids assay:

Test Compound

Concentration

Tested (ng/mL)

Ketoconazole

1,000,000

The following compounds do not cross-react above the sensitivity (13.00 ng/mL) of the AxSYM Cannabinoids assay (Compounds tested at 100,000 ng/mL, unless otherwise indicated):

Acetaminophen	Lovastaten
Acetylsalicylic Acid	Melanin
Alprazolam	Meperidine
Amitryptyline	Methadone
Amobarbital	Methadone Primary Metabolite (EDDP)
Amoxicillin
d, l Ð Amphetamine	d-Methamphetamine
Ampicillin	3,4 Ð Methylenedioxyamphetamine (MDA)
Aspartame	3,4 Ð Methylenedioxy-N-ethylamphetamine (MDE)
Benzocaine	3,4 Ð Methylenedioxy-methamphetamine (MDMA)
Benzoic Acid	Methylphenidate
Benzoylecgonine	Methyprylon
Buprenorphine*	Morphine
Butabarbital	Naloxone
Caffeine	Naltrexone
Calcium Hypochlorite	Naproxen*
Chloramphenicol	Niacinamide
Chlordiazepoxide	Nicotine
Chloroquine	Norfloxacin*
Chlorpheniramine*	Oxycodone
Cholesterol	Oxymorphone*
Ciprofloxacin*	Penicillin G*
Clindamycin	Phenethylamine
Cocaine	Phenobarbital
Codeine	Phenothiazine
Diazepam	Phentermine
Digoxin	Phenylbutazone
Dihydrocodeine	Phenylpropanolamine
Dihydromorphine	Progesterone#
Diphenhydramine	Prolintane
Ecgonine	Promethazine*
Ephedrine	Pseudoephedrine
Epinephrine	Quinine
Erythromycin*	Salicyclic Acid
Estriol	Secobarbital
Fenoprofen*	Serotonin
Furosemide*	Sulindac
Gentisic Acid	Tetracycline
Glutethimide	Theophylline
Guaiacol Glycerel Ether	Tolbutamide
Haloperidol	Trifluoperazine
Histamine	Trimethoprim
Hydralazine	Tyramine
Hydrocodone
Hydromorphone
Ibuprofen*
Isoproteronol
Ketamine*
Ketoprofen

# Tested at 10,000 ng/mL

* Tested at 1,000,000 ng/mL

Interference

The following compounds added to human urine, resulted in less than 10% error in detecting added drug when assayed with the AxSYM Cannabinoids assay.

Compound	Concentration Tested
Acetone	1	g/dL
Ascorbic Acid	1.5	g/dL
Bilirubin	0.25	mg/dL
Creatinine	500	mg/dL
Ethanol	1	g/dL
Glucose	2	g/dL
Hemoglobin (lysed red blood cells)	115	mg/dL
Oxalic Acid	100	mg/dL
Riboflavin	7.5	mg/dL
Sodium Chloride	6	g/dL
Total Protein	0.05	g/dL
Urea	6	g/dL

The presence of detergents in samples potentially interferes with immunoassay results.

Bleach (hypochlorite) is a strong oxidizing agent. As a consequence, bleach may cause oxidation of some analytes, thus reducing the amount available for analysis. This may result in lower analytic results, regardless of the methodology.

There is the possibility that other substances and/or factors not listed above may interfere with the test and cause false results.

REFERENCE

1. Abbott AxSYM Cannabinoids package insert, October 1997