Item Value
Test name Central Blood Culture
Available Stat? No
Turn around times Up to 6 days
In House Availability Daily, all shifts
Performed by Microbiology
Normal range No growth
Container type Paired blood culture bottles (BD BACTEC Plus Aerobic and Lytic Anaerobic bottles)
Amount to Collect Adults: 20 mL total (10 mL for each bottle)
Pediatrics: Collect blood sample amount according to weight:
< 1 kg = 1 mL for aerobic only (0.5 ml for neonates < 72h old)
1 – 5 kg = 2 mL total (1 mL for each bottle)
5 – 15 kg = 3 mL total (1.5 mL for each bottle)
15 – 40 kg = 6 mL total (3 mL for each bottle)
>40 kg = 10 mL total (5 mL for each bottle)
Collection Instructions 1. Adults:
Collect 2 sets of cultures from different sites.
Collect at least one set of cultures from a peripheral site.

2. Pediatrics:
Amount of blood depends on weight of patient (see weight based minimums).

Anaerobic sample should be sent only in the following circumstances: Outpatients, patients with immunodeficiency, malignancy or after bone or human stem cell transplant, patients with gastrointestinal disorder, or at physician's request due to concern for anaerobes. Sending only an aerobic specimen is sufficient for all other patient populations.

3. Clarify which line(s) to obtain sample from with the provider.

4. Remove plastic cap of each bottle and scrub top of each bottle with 70% alcohol prep pad.

5. Obtain sample. Instill sample into blood culture bottles. Refer to Blood Culture Methods (General) procedure in Nursing Procedures Manual.

6. Instill sample into aerobic bottle first and then into anaerobic bottle. Do not aspirate air into the anaerobic bottle. Do not add more than 10 mL into each bottle. Gently invert bottles to mix contents.

7. Label each bottle with patient's name and medical record number, type of central line, and indicate location of the line and the lumen color, if desired. Do not place label on neck of bottle or bottom (underneath) of bottle, and do not cover bar code on bottle with the label. Place label vertically on bottle.
Sample type Blood

If you have additional questions regarding this test, please call: 415-353-1667