Item Value
Test name Bacterial Culture, Normally Sterile Site, with Gram stain
Available Stat? No
Turn around times Up to 14 days
In House Availability Set up daily, day and evening shifts
Performed by Microbiology
Normal range No growth
Container type
Peritoneal fluid Aerobic and anaerobic blood culture bottles, and sterile tube
Non-peritoneal fluid Sterile tube
Tissue Anaerobic transport vial or sterile container
Bone marrow aspirate 1.5 mL Isolator tube
Swabs Amies transport medium with charcoal
Amount to Collect
Peritoneal fluid 25 mL
Non-peritoneal fluid 25 mL
Tissue 5 cu mm
Bone marrow aspirate 0.5 mL
Collection Instructions Submitting peritoneal fluid
(abdominal fluid, ascitic fluid, dialysis fluid) for bacterial culture:

1. inoculate 10 ml into each of aerobic and anaerobic blood culture bottles at the patient's bedside for optimal growth and recovery of organisms. Divide the fluid equally among the bottles if < 20 mL is obtained.

2. Submit additional fluid in a sterile tube, which allows for gram stain to be performed and faster identification of bacteria present at high concentrations.

Submitting tissue
For tissues not collected in the OR, obtain anaerobic transport medium from Microbiology L553 or from the Mount Zion OR

Submitting bone marrow aspirate
Schedule in advance at the time the bone marrow is requested.
Sample type Body fluid, unfixed tissue from normally sterile sites, bone marrow aspirate

If you have additional questions regarding this test, please call: 415-353-1667