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Lab Manual for Moffitt-Long and Mount Zion

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White Blood Cell Differential (Complete View)

Item Value
Test name White Blood Cell Differential
Test Update Information
Approval req'd? No
Available Stat? Yes
Utilization Guidelines
Test code CBCD, WBCDF
Test group
Performed by Parnassus & Mt. Zion Hematology
Sendout?
In House Availability Test available 24 hours per day 7 days per week
Method
Patient Preparation
Collection Instructions
Container type Lavender top
Amount to Collect 3 mL blood
Sample type EDTA whole blood
Preferred volume 3 mL blood
Min. Volume 1 mL blood (or 250 µL in a pedi-bullet)
UCSF Rejection Criteria
Processing notes
Ref Lab Rejection Criteria
Units x109/L
Normal range Normal Absolute Counts in x109/L (95% confidence interval):

AGE NEUTS LYMPH MONO EO BASO
0-24 hours 5.0-28 2.0-12 0.4-3.1 0.0-0.9 0.0-0.6
24 hours-1 wk 1.5-21 2.0-17 0.3-2.7 0.1-1.1 0.0-0.3
1 wk-6 months 1.0-10 2.0-17 0.2-2.7 0.1-1.1 0.0-0.3
6 months -4 years 1.0-8.5 2.0-14 0.0-0.9 0.0-1.1 0.0-0.3
4-14 years 1.5-8.5 1.2-8.0 0.0-1.4 0.0-1.1 0.0-0.3
14-21 years 1.8-8.0 1.0-6.1 0.0-1.4 0.0-0.8 0.0-0.3
> 21 years 1.8-6.8 1.0-3.4 0.2-0.8 0.0-0.4 0.0-0.1
Critical value Neutrophils ≤ 1.0 x109/L

Note: Neutrophil counts ≤ 1.0 x109/L are not called if a prior critical was reported in the preceding 24 hours
Synonyms Eosnophil; Basophil; Neutrophiil; Granulocyte; Monocyte; Lymphocyte; Leukocyte differential; WBC differential
Stability
Turn around times Stat 1 hour, Routine 4 hours
Reflex?
Additional information All differential counts are routinely performed by automated flow cytometry.

Total neutrophils, both mature PMNs and immature forms, are counted. The presence of immature granulocytes/left shift (metamyelocytes and myelocytes) is reported, but not quantitated. Smears with this finding will be reviewed microscopically if a left shift has not already been noted within the previous 72 hours.

A manual differential will be initiated by the laboratory at a separate charge if an automated differential is technically inadequate for the analysis of a particular sample.

Manual differentials also include RBC morphology and platelet morphology.
CPT coding
Medical Necessity?
LOINC code
LDT or Mod FDA?
Include for internal use?
Last Updated 6/7/2013 10:50:26 AM
Entry Number 305
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