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Lab Manual for Moffitt-Long and Mount Zion

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Vitamin B6

Item Value
Approval req'd? No
Available Stat? No
Test code VIB6
Performed by Quest
Sendout? Yes
Method Liquid Chromatography, Tandem Mass Spectrometry
Patient Preparation Patient follows an overnight fast, and restricted from alcohol and vitamins for at least 24 hrs.
Collection Instructions Wrap the collection tube in aluminum foil to protect it from light during transport to the laboratory. If separation of cells can't be performed immediately after collection, keep the whole blood refrigerated (on ice) and protect from light. The separation of cells must be completed within 6 hours.
Container type Lavender top (on ice) (*Green top on ice acceptable)
Amount to Collect 2 mL blood
Sample type EDTA plasma
Preferred volume 1 mL plasma
Min. Volume 0.5 mL plasma
UCSF Rejection Criteria Specimens left at room temperature over 6 hrs. Collected in tubes other than lavender top tubes.
Processing notes Centrifuge under refrigeration at 2-8 C for 5-10 minutes, transfer plasma into a dark brown transport tube or wrapped in aluminum foil. Freeze the plasma at -20C and ship frozen to China Basin sendouts. Order Quest test # 926.
Ref Lab Rejection Criteria Hemolysis, Lipemia, thawed plasma, specimens not protected from light. Shipped/store at refrigerated or room temperatures. Specimens left at room temperature over 6 hours. Collected in tubes other than lavender top tubes.
Units ng/mL
Normal range
2-17 years 3.0-35 ng/mL
> 17 years 2.1-21.7 ng/mL
Synonyms Pyridoxine
Stability Room temperature 6 hours, refrigerated 12 hours, frozen at -20C 6 days, frozen at -70C 6 weeks
Turn around times Set up 5 days per week. Turnaround time 4-5 days
Additional information To convert ng/mL to nmol/L (SI units) multiply by 4.046.

Pyridoxine is the molecule most commonly referred to as 'Vit. B6', Pyridoxal-5'-phosphate is the active form of the vitamin.
CPT coding 84207-90
LOINC code 30552-4
Last Updated 2/20/2013 4:16:59 PM
Entry Number 999

If you have additional questions regarding this test, please call: 415-353-1667

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