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Shiga Toxin Assay

Item Value
Approval req'd? No
Available Stat? No
Test code T315
Performed by State Microbial Disease Laboratory
Sendout? Yes
In House Availability Sent to state lab Monday-Friday day shift only.
Method Tissue culture
Collection Instructions Submit stool to laboratory within 3 hours of collection.

If specimen will be submitted to the laboratory more than 3 hours after collection, or after 11 pm when Microbiology is closed, submit stool in C & S (Cary-Blair) Medium collection vial. Add stool to red line on vial and mix well with spoon. Obtain vials from ACC phlebotomy stations at A 122 or A 220A or from the Emergency Dept.
Container type Clean container or Cary-Blair transport media
Sample type Fresh stool
Preferred volume 8 gm or 8 mL
Min. Volume 8 gm or 8 mL
UCSF Rejection Criteria Improperly submitted samples.
Processing notes If sample is submitted unpreserved, place in Cary-Blair vial. Refrigerate Cary-Blair vials
Normal range No toxin detected
Critical value Shiga Toxin detected
Synonyms Hemolytic uremic syndrome; HUS; verocytotoxin
Stability Unpreserved 3 hours, preserved 1 week.
Turn around times Minimum 2 weeks
Additional information The stool sample is sent out for this test when E. coli 0157 culture is requested or when a bloody stool is received for bacterial culture.
LOINC code 29257-3
Last Updated 10/18/2012 12:27:09 PM
Entry Number 983

If you have additional questions regarding this test, please call: 415-353-1667

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