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Shiga Toxin Assay

Item Value
Approval req'd? No
Available Stat? No
Test code P315
Performed by Microbiology
In House Availability Performed once per day on day shift only.
Method Rapid membrane enzyme immunoassay
Collection Instructions Submit unpreserved stool to laboratory within 3 hours of collection.

If specimen will be submitted to the laboratory more than 3 hours after collection, or after 11 pm when Microbiology is closed, submit stool in C & S (Cary & Blair) Medium. Add stool to red line on vial and mix well with spoon.

C & S (Cary & Blair) Medium is available from Material Services . Outpatients can obtain these from the laboratories' draw stations.
For patient collect samples, order PMM 68902 C & S Medium Cary Blair 2805-05-WB (with bag and instructions).
PMM 49206 C & S Medium 2805-05 (without bag or instructions) also available.

Two specimens collected 24-48 hours apart are sufficient for the detection of the great majority of pathogens.
Container type Urine cup or C & S (Cary & Blair) transport media
Amount to Collect 5 mL
Sample type Stool
Preferred volume 5 mL
Min. Volume 5 mL
UCSF Rejection Criteria Improperly submitted samples.
Normal range Not detected
Critical value Shiga Toxin detected
Synonyms Hemolytic uremic syndrome; HUS; verocytotoxin
Stability Unpreserved 3 hours, preserved and refrigerated 2 weeks
Turn around times 2 days
Reflex? Culture for E. coli O157 and Shiga Toxin Assay is automatically performed on stools submitted for bacterial culture, and are billed separately.
Additional information This test is for the simultaneous qualitative detection and differentiation of Shiga toxin 1 and Shiga toxin 2.
CPT coding 87427 x2
LOINC code 29257-3
Last Updated 1/20/2016 11:05:36 AM
Entry Number 983
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