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Shiga Toxin Assay
|In House Availability||Performed once per day on day shift only.|
|Method||Rapid membrane enzyme immunoassay|
|Collection Instructions||Submit unpreserved stool to laboratory within 3 hours of collection.
If specimen will be submitted to the laboratory more than 3 hours after collection, or after 11 pm when Microbiology is closed, submit stool in C & S (Cary & Blair) Medium. Add stool to red line on vial and mix well with spoon.
C & S (Cary & Blair) Medium is available from Material Services . Outpatients can obtain these from the laboratories' draw stations.
For patient collect samples, order PMM 68902 C & S Medium Cary Blair 2805-05-WB (with bag and instructions).
PMM 49206 C & S Medium 2805-05 (without bag or instructions) also available.
|Container type||Urine cup or C & S (Cary & Blair) transport media|
|Amount to Collect||5 mL|
|Preferred volume||5 mL|
|Min. Volume||Fresh stool : 0.5 mL or size of pea, Stool in C & S (Cary & Blair) transport medium: 5 mL|
|UCSF Rejection Criteria||Unpreserved stool received > 3 hours after collection. More than two samples per day.|
|Processing notes||If < 5mL stool received, add 3 parts Cary & Blair medium to 1 part stool.|
|Normal range||Not detected|
|Critical value||Shiga Toxin detected|
|Synonyms||Hemolytic uremic syndrome; HUS; verocytotoxin|
|Stability||Unpreserved 3 hours, preserved and refrigerated 2 weeks|
|Turn around times||2 days|
|Reflex?||Culture for E. coli O157 and Shiga Toxin Assay is automatically performed on stools submitted for bacterial culture, and are billed separately.|
|Additional information||Bacterial Culture-Stool, E. coli O157 Culture, and Shiga Toxin Assay are orderable as a panel in Apex (Community-Acquired Diarrhea Testing Panel). If testing for individual components of this panel is desired, please contact the microbiology lab.
This test is for the simultaneous qualitative detection and differentiation of Shiga toxin 1 and Shiga toxin 2.
|CPT coding||87427 x2|
|Last Updated||10/13/2016 5:39:21 PM|