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Shiga Toxin Assay

Item Value
Approval req'd? No
Available Stat? No
Test code P315
Performed by Microbiology
In House Availability Performed once per day on day shift only.
Method Rapid membrane enzyme immunoassay
Collection Instructions Submit unpreserved stool to laboratory within 3 hours of collection.

If specimen will be submitted to the laboratory more than 3 hours after collection, or after 11 pm when Microbiology is closed, submit stool in C & S (Cary & Blair) Medium. Add stool to red line on vial and mix well with spoon.

C & S (Cary & Blair) Medium is available from Material Services . Outpatients can obtain these from the laboratories' draw stations.
For patient collect samples, order PMM 68902 C & S Medium Cary Blair 2805-05-WB (with bag and instructions).
PMM 49206 C & S Medium 2805-05 (without bag or instructions) also available.

Container type Urine cup or C & S (Cary & Blair) transport media
Amount to Collect 5 mL
Sample type Stool
Preferred volume 5 mL
Min. Volume Fresh stool : 0.5 mL or size of pea, Stool in C & S (Cary & Blair) transport medium: 5 mL
UCSF Rejection Criteria Unpreserved stool received > 3 hours after collection. More than two samples per day.
Processing notes If < 5mL stool received, add 3 parts Cary & Blair medium to 1 part stool.
Normal range Not detected
Critical value Shiga Toxin detected
Synonyms Hemolytic uremic syndrome; HUS; verocytotoxin
Stability Unpreserved 3 hours, preserved and refrigerated 2 weeks
Turn around times 2 days
Reflex? Culture for E. coli O157 and Shiga Toxin Assay is automatically performed on stools submitted for bacterial culture, and are billed separately.
Additional information Bacterial Culture-Stool, E. coli O157 Culture, and Shiga Toxin Assay are orderable as a panel in Apex (Community-Acquired Diarrhea Testing Panel). If testing for individual components of this panel is desired, please contact the microbiology lab.

This test is for the simultaneous qualitative detection and differentiation of Shiga toxin 1 and Shiga toxin 2.
CPT coding 87427 x2
LOINC code 29257-3
Last Updated 10/13/2016 5:39:21 PM
Entry Number 983
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