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Shiga Toxin Assay
|In House Availability||Performed once per day on day shift only.|
|Method||Rapid membrane enzyme immunoassay|
|Collection Instructions||Submit unpreserved stool to laboratory within 3 hours of collection.
If specimen will be submitted to the laboratory more than 3 hours after collection, or after 11 pm when Microbiology is closed, submit stool in C & S (Cary & Blair) Medium. Add stool to red line on vial and mix well with spoon.
C & S (Cary & Blair) Medium is available from Material Services . Outpatients can obtain these from the laboratories' draw stations.
For patient collect samples, order PMM 68902 C & S Medium Cary Blair 2805-05-WB (with bag and instructions).
PMM 49206 C & S Medium 2805-05 (without bag or instructions) also available.
Two specimens collected 24-48 hours apart are sufficient for the detection of the great majority of pathogens.
|Container type||Urine cup or C & S (Cary & Blair) transport media|
|Amount to Collect||5 mL|
|Preferred volume||5 mL|
|Min. Volume||5 mL|
|UCSF Rejection Criteria||Improperly submitted samples.|
|Normal range||Not detected|
|Critical value||Shiga Toxin detected|
|Synonyms||Hemolytic uremic syndrome; HUS; verocytotoxin|
|Stability||Unpreserved 3 hours, preserved and refrigerated 2 weeks|
|Turn around times||2 days|
|Reflex?||Culture for E. coli O157 and Shiga Toxin Assay is automatically performed on stools submitted for bacterial culture, and are billed separately.|
|Additional information||This test is for the simultaneous qualitative detection and differentiation of Shiga toxin 1 and Shiga toxin 2.|
|CPT coding||87427 x2|
|Last Updated||1/20/2016 11:05:36 AM|