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Bacterial Culture, Stool

Item Value
Approval req'd? Yes, consultation required for samples submitted > 72 hours after inpatient admission. Contact Microbiology at x31268
Available Stat? No
Test code P166
Test group Bacterial Culture
Performed by Microbiology
In House Availability Set up daily on day and evening shifts
Method Culture
Collection Instructions Submit stool to laboratory within 3 hours of collection.

If specimen will be submitted to the laboratory more than 3 hours after collection, or after 11 pm when Microbiology is closed, submit stool in C & S (Cary-Blair) Medium collection vial. Add stool to red line on vial and mix well with spoon. Obtain vials from outpatient phlebotomy stations or from the Microbiology Lab.

Two specimens collected 24-48 hours apart are sufficient for the detection of the great majority of pathogens
Container type Urine cup or Cary Blair transport medium
Amount to Collect 5 mL
Sample type Stool
Preferred volume 5 mL
Min. Volume 5 mL
UCSF Rejection Criteria Unpreserved stool received > 3 hours after collection. More than two samples per episode or more than one sample received within 24 hours
Normal range
Critical value Stools positive with E. coli O157:H7, Vibrio cholerae or Salmonella sp. that cause enteric fever (e.g. S. typhi). Stools positive for Shiga toxin producing E.coli.
Synonyms Salmonella; Shigella; Campylobacter; Vibrio
Stability Unpreserved 3 hours, preserved 1 week.
Turn around times 3-5 days
Reflex? If bacteria are detected that are not normal mixed flora they are automatically identified and susceptibility testing is performed if appropriate.

Susceptibility testing may be omitted upon request when sample is submitted for testing.

Culture for E. coli O157 and Shiga Toxin Assay is automatically performed on stools submitted for bacterial culture, and are billed separately.
Additional information Unusual findings such as the absence of normal gram-negative enteric flora are reported.

If suspected Yersinia species [P158] and Vibrio species [P159] must be requested separately to ensure that selective media are inoculated,

C. difficile is not cultured. If C. difficile is suspected request the C. difficile antigen assay (P328).
CPT coding 87045, 87046
LOINC code 625-4
Last Updated 2/19/2014 2:56:28 PM
Entry Number 98
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