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Bacterial Culture, Stool
|Approval req'd?||Yes, consultation required for samples submitted > 72 hours after inpatient admission. Contact Microbiology at x31268|
|Test group||Bacterial Culture|
|In House Availability||Set up daily on day and evening shifts|
|Collection Instructions||Submit unpreserved stool to laboratory within 3 hours of collection.
If specimen will be submitted to the laboratory more than 3 hours after collection, or after 11 pm when Microbiology is closed, submit stool in C & S (Cary & Blair) Medium. Add stool to red line on vial and mix well with spoon.
C & S (Cary & Blair) Medium is available from Material Services . Outpatients can obtain these from the laboratories' draw stations.
For patient collect samples, order PMM 68902 C & S Medium Cary Blair 2805-05-WB (with bag and instructions).
PMM 49206 C & S Medium 2805-05 (without bag or instructions) also available.
|Container type||Urine cup or C & S (Cary & Blair) transport medium|
|Amount to Collect||5 mL|
|Preferred volume||5 mL|
|Min. Volume||Fresh stool : 0.5 mL or size of pea, Stool in C & S (Cary & Blair) transport medium: 5 mL|
|UCSF Rejection Criteria||Unpreserved stool received > 3 hours after collection. More than two samples per day.|
|Processing notes||If < 5mL stool received, add 3 parts Cary & Blair medium to 1 part stool.|
|Critical value||Stools positive with E. coli O157:H7, Vibrio cholerae or Salmonella sp. that cause enteric fever (e.g. S. typhi). Stools positive for Shiga toxin producing E.coli.|
|Synonyms||Salmonella; Shigella; Campylobacter; Vibrio|
|Stability||Unpreserved 3 hours, preserved 1 week.|
|Turn around times||3-5 days|
|Reflex?||If bacteria are detected that are not normal mixed flora they are automatically identified and susceptibility testing is performed if appropriate.
Susceptibility testing may be omitted upon request when sample is submitted for testing.
Culture for E. coli O157 and Shiga Toxin Assay is automatically performed on stools submitted for bacterial culture, and are billed separately.
|Additional information||Bacterial Culture-Stool, E. coli O157 Culture, and Shiga Toxin Assay are orderable as a panel in Apex (Community-Acquired Diarrhea Testing Panel). If testing for individual components of this panel is desired, please contact the microbiology lab.
Unusual findings such as the absence of normal gram-negative enteric flora are reported.
If suspected Yersinia species [P158] and Vibrio species [P159] must be requested separately to ensure that selective media are inoculated,
C. difficile is not cultured. If C. difficile is suspected request the C. difficile antigen assay (P328).
|CPT coding||87045, 87046|
|Last Updated||10/13/2016 5:15:34 PM|