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Trypanosome Exam

Item Value
Approval req'd? No, but contact parasitologist in Microbiology (x31268) prior to specimen collection
Available Stat? No
Test code P409
Test group Trypanosoma
Performed by Microbiology
Sendout? Physician may also request Microbiology to send sample to CDC for T. cruzi PCR
In House Availability Monday-Thursday before 12:30 pm
Method Microscopy (Giema stain of thick and thin smears)
Collection Instructions Collect blood prior to anti-parasitic therapy.

Contact parasitologist in Microbiology (x31268) prior to collection to discuss appropriate sample type and collection instructions.

Collect and deliver the specimen directly to the laboratory within 30 minutes of collection Monday-Thursday before 12:30 pm.

Include travel/residence history on requisition.
Container type
Blood Lavender or Dark Green top
CSF CSF tube or sterile collection tube
Heart biopsy for CDC PCR Testing Sterile collection tube with saline or FFPE material
Amount to Collect
Blood 3 mL
CSF 1 mL
Heart biopsy for CDC PCR Testing 3 cubic mm (fresh or from FFPE material)
Sample type EDTA whole blood, CSF
Heart biopsy in sterile saline or FFPE
Preferred volume
Blood 3 mL
CSF 1 mL
Heart biopsy for CDC PCR Testing 3 cubic mm (fresh or from FFPE material)
Min. Volume
Blood (Adult) 2.5 mL
Blood (Pediatric) 0.5 mL
CSF 0.5 mL
Heart biopsy for CDC PCR Testing 2.5 cubic mm (fresh or from FFPE material)
Processing notes Store samples in refrigerator. Send to Microbiology refrigerated

For PCR to CDC:
Ship Monday-Thursday, overnight to avoid weekend deliveries. Ship sample on cold packs, except ship paraffin embedded tissue at ambient temperature.

Request provider to complete page 2 of CDC Specimen Submission Form 50.34, which is available at http://www.cdc.gov/laboratory/specimen-submission/form.html
Normal range No Trypanosoma species seen/detected
Critical value Positive result from normally sterile sites
Synonyms Trypanosoma cruzi, Chagas disease
Stability Rerfrigerated 24 hours
Turn around times 1-3 days
Additional information This test is offered for the causitive agent of Chagas disease. Sensitivity of direct smear is limited to severe acute stage, reactivation, or detection in recipients of organs from donors with chronic Chagas disease.

Physician may also request Microbiology to send sample to CDC for T. cruzi PCR

For African trypanosomiasis (sleeping sickness), thick and thin smears of blood, CSF, or aspirates of lymph node and trypanosomal chancres can be examined.

CDC offers clinical consultation, but not testing, for suspect African trypanosomiasis.
CPT coding 87207
Last Updated 11/23/2015 11:05:45 AM
Entry Number 954
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