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Thyroid Stimulating Hormone

Item Value
Test Update Information Effective May 1, 2016, TSH testing will be performed on the Abbott Architect i2000 analyzer. TSH values read approximately 10% lower than the prior Beckman DxI method but did not significantly affect the reference range or classification of subjects as normal or abnormal.
Clinical Questions Does my patient have autoimmune thyroiditis?
Approval req'd? No
Available Stat? No
Test code TSH
Test group Thyroid tests
Performed by Chemistry China Basin
In House Availability Test performed Monday-Saturday (day shift)
Method Chemiluminescent Microparticle Immunoassay (Abbott Architect i2000)
Container type Gold top or Light Green top
Amount to Collect 1.6 mL blood
Sample type Serum or heparinized plasma
Preferred volume 0.6 mL serum or heparinized plasma
Min. Volume 0.4 mL serum or heparinized plasma
Processing notes Refrigerate.
Units mIU/L
Normal range
Age Reference range (mIU/L)
<2 months 1.12-6.31
2 months to <6 months 0.73-4.77
6 months to <14 years 0.70-4.17
14 years to <18 years 0.47-3.41
18 years and above 0.45-4.12


Reference ranges apply to non-pregnant individuals. In pregnant subjects, TSH levels decline in the first trimester and then rise after 10 -12 weeks gestation. The upper reference limit is 2.5 mIU/L in the first trimester, 3.0 in the second trimester, and 3.5 mIU/L in the third trimester. The lower reference limit for TSH in pregnancy is 0.1 – 0.2 mIU/L lower than the range limit in non-pregnant subjects (Garber JR et al. Clinical Practice Guidelines for Hypothyroidism in Adults: American Association of Clinical Endocrinologists and the American Thyroid Association, Thyroid 12:1200-1235, 2012).

Pediatric reference ranges adopted from Pediatric Reference Intervals seventh edition (Soldin, Steven J. et al) and the Canadian Laboratory Initiative on Reference Interval Database (CALIPER) study, Clinical Chemistry September 2013 vol. 59 no. 9 1393-1405.

Neonatal and cord blood levels are 2-4x higher than levels at ≥ 2 weeks of age through adult life. Neonatal levels are also screened by the State program.

Adult reference ranges were adopted from NHANES III and verified in 63 adult blood donors (Hollowell JG et al. Serum TSH, T4, and thyroid antibodies in the United States population ,1988 to 1994: National Health and Nutrition Examination Survey , NHANES III. J Clin Endocrinol Metab 87:489–499, 2002. Garber JR et al. Clinical Practice Guidelines for Hypothyroidism in Adults: American Association of Clinical Endocrinologists and the American Thyroid Association, Thyroid 12:1200-1235, 2012).
Synonyms TSH; Thyrotropin
Turn around times 0 -2 days
Additional information According to the assay manufacturer the low end functional sensitivity (20% coefficient of variation cutoff) for this assay is 0.01 mIU/L.
CPT coding 84443
LOINC code 3016-3
Last Updated 5/5/2016 5:08:12 PM
Entry Number 924
Lab Procedure Link Click here for Procedure
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