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|Performed by||Parnassus Hematology|
|Collection Instructions||Do not draw from heparin-containing lines.
1. Check the expiration date on the label of the blue top vacutainer before drawing the patient.
2. For blood collection in a sodium citrate blue top, the tube must be filled to above the Minimum Fill Indicator on the tube. It is crucial to wait and allow the tube to stop filling before removing it from the needle.
3. With use of a butterfly needle, draw about 1 cc using a separate blue top to remove air from tubing, discard the first tube and then draw a second blue top tube filled to the full extent of the vacuum.
4. Tubes should not be filled past the Maximum Fill dashed line by either using a syringe or removing the tube cap.
For patients with Hct's ≥ 55% please contact Hematology (x3-1747) to obtain blue top tubes with adjusted citrate volumes in order to maintain the proper citrate to plasma ratio for coagulation studies.
|Container type||Blue top filled to full extent of vacuum|
|Amount to Collect||2.7 mL blood|
|Sample type||Citrated plasma|
|Preferred volume||1 mL plasma|
|UCSF Rejection Criteria||Samples collected in outdated blue top vacutainer. Over-filled or under-filled tubes may be rejected|
|Processing notes||Test specimens within four hours of collection or freeze plasma in a plastic tube at -20C.|
|Normal range||21.6-30.1 sec|
|Turn around times||Test run 0800-1600 daily. Turnaround: 4 hours|
|Additional information||A prolonged thrombin time can be due to inhibitors of thrombin (for example, unfractionated heparin, direct thrombin inhibitors including argatroban and lepirudin, or paraneoplastic-associated heparinoid) or fibrinogen abnormalities (afibrinogenemia, hypofibrinogenemia, dysfibrinogenemia, anti-fibrinogen antibodies, or high fibrin split products).Correlation with fibrinogen level and clinical setting is suggested. Laboratory medicine resident can be reached by contacting the hematology lab at 353-1747.|
|Last Updated||8/7/2012 2:04:15 PM|
If you have additional questions regarding this test, please call: 415-353-1667