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Lab Manual for Moffitt-Long and Mount Zion

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Testosterone, Free (includes Total Testosterone)

Item Value
Approval req'd? No
Available Stat? No
Test code FTST
Test group Testosterone
Performed by Quest
Sendout? Yes
Method LC/MS/MS, Tracer Equilibrium Dialysis, Calculation
Container type Red top (Gold top NOT acceptable )
Amount to Collect 2 mL blood
Sample type Serum
Preferred volume 1 mL serum
Min. Volume 0.4 mL serum
UCSF Rejection Criteria Collected in Gold top. Grossly hemolyzed samples.
Processing notes Refrigerate serum. Specify age and sex on test request form. Order Quest # 36170
Units ng/dL and pg/mL
Normal range Testosterone, Total:

Age Males Females
Cord Blood 17-61 ng/dL 16-44 ng/dL
1-10 days ≤ 187 ng/dL ≤ 24 ng/dL
1-3 months 72-344 ng/dL ≤ 17 ng/dL
3-5 months ≤ 201 ng/dL ≤ 12 ng/dL
5-7 months ≤ 59 ng/dL ≤ 13 ng/dL
7-12 months ≤ 16 ng/dL ≤ 11 ng/dL
1-5.9 years ≤ 5 ng/dL ≤ 8 or less
6-7.9 years ≤ 25 ng/dL ≤ 20 or less
8-10.9 years ≤ 42 ng/dL ≤ 35 or less
11-11.9 years ≤ 260 ng/dL ≤ 40 ng/dL
12-13.9 years ≤ 420 ng/dL ≤ 40 ng/dL
14-17.9 years ≤ 1000 ng/dL ≤ 40 ng/dL
18-69 years 250 - 1100 ng/dL 2 - 45 ng/dL
70-89 years 90 - 890 ng/dL 2 - 40 ng/dL


Pediatric Reference Ranges by Pubertal Stage :
Stages Males Females
I ≤ 5 ng/dL ≤ 8 ng/dL
II ≤ 167 ng/dL ≤ 24 ng/dL
III 21-719 ng/dL ≤ 28 ng/dL
IV 25-912 ng/dL ≤ 31 ng/dL
V 110-975 ng/dL ≤ 33 ng/dL


Testosterone, Free:

Age Males Females
5-9.9 years ≤ 5.3 pg.mL 0.2-5.0 pg.mL
10-13.9 years 0.7-52.0 pg/mL 0.1-7.4 pg/mL
18-69 years 35.0-155.0 pg/mL 0.1-6.4 pg/mL
70-89 years 30.0-135.0 pg/mL 0.2-3.7 pg/mL
14-17.9 years 18.0-111.0 pg/mL 0.5-3.9 pg/mL
Synonyms Free testosterone
Stability Room temperature 1 week, refrigerated 1 week, frozen at -20C 2 years.
Turn around times Test run daily. Turnaround time: 6-8 days.
Additional information Includes determination of Total Testosterone.

To convert ng/L to pmol/L (SI units) multiply by 3.47.

Newborn and 5-7 months old reference ranges are taken from Tietz, NW, Clinical Guide to Laboratory Tests, 2nd ed., 1990, Saunders, Philadelphia.
CPT coding 84402-90, 84403-90
LOINC code 2991-8
Last Updated 10/28/2013 11:48:58 AM
Entry Number 891

If you have additional questions regarding this test, please call: 415-353-1667

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