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Serotonin Release Assay
| Item | Value |
|---|---|
| Approval req'd? | Yes. If SRA is required before result of heparin-PF4 complex antibody test is available, please contact hematology at x3-1747 |
| Available Stat? | No |
| Test code | SRAU |
| Test group | HIT |
| Performed by | Quest |
| Sendout? | Yes |
| Method | Radiobinding 14C |
| Patient Preparation | The patient must be off heparin for 24 hours before drawing specimen for SRA. |
| Collection Instructions | A Heparin-PF4 Complex Antibody test (HPA) should be performed prior to requesting a Serotonin Release Assay (SRA), and the SRA should only be performed if clinically indicated. Note: This test is not authorized or offered for B&T patients, for whom the in-house assay for HPA should be ordered. |
| Container type | Red top |
| Amount to Collect | 1 full red top (may not be combined with other tests) |
| Sample type | Serum |
| Preferred volume | 1 mL serum |
| Min. Volume | 0.5 mL serum |
| UCSF Rejection Criteria | Hemolyzed or lipemic samples. |
| Processing notes | Deliver tube to Hematology asap for processing. Freeze serum at -20C. Order Quest test #14627. |
| Units | % release at 3 doses of UFH (0.1 IU/mL, 0.35 IU/mL and 100 IU/mL) |
| Normal range | Negative |
| Critical value | Quest Priority-2: ≥ 20 % release |
| Synonyms | HIT; heparin induced thrombocytopenia; SRA; unfractionated heparin |
| Turn around times | Test performed once a week. Turnaround time: 10-14 days. |
| Additional information | A negative test does not exclude thrombotic risk due to heparin induced thrombocytopenia (HIT), nor does a positive test exclude other causes of thrombocytopenia. Correlation with heparin-PF4 complex antibody test, platelet counts, and clinical finding can be important for diagnosis of HIT. A Heparin-PF4 Complex Antibody test (HPA) should be performed prior to requesting a Serotonin Release Assay (SRA). If an SRA is requested prior to the result of HPA testing being completed, the following text will be sent: "Test not performed. If clinically indicated, after results of heparin-platelet factor 4 antibody test (HPA) are available, please re-order SRA. It is generally recommended that patient not receive heparin for 24 hours prior to SRA testing." The SRA is often not necessary in the presence of a positive test for heparin-PF4 antibodies. |
| CPT coding | 86022-90 |
| LOINC code | 50735-0 |
| Last Updated | 4/14/2011 2:43:18 PM |
| Entry Number | 851 |
If you have additional questions regarding this test, please call: 415-353-1667