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If you have additional questions regarding this test, please call: 415-353-1667
Serotonin Release Assay
|Clinical Questions||Does my patient have drug-induced thrombocytopenia (including HIT)?|
|Approval req'd?||Yes. If SRA is required before result of heparin-PF4 complex antibody test is available, please contact hematology at x3-1747|
|Patient Preparation||The patient must be off heparin for 24 hours before drawing specimen for SRA.|
|Collection Instructions||A Heparin-PF4 Complex Antibody test (HPA) should be performed prior to requesting a Serotonin Release Assay (SRA), and the SRA should only be performed if clinically indicated.
Note: This test is not authorized or offered for B&T patients, for whom the in-house assay for HPA should be ordered.
|Container type||Red top|
|Amount to Collect||1 full red top (may not be combined with other tests)|
|Preferred volume||1 mL serum|
|Min. Volume||0.5 mL serum|
|UCSF Rejection Criteria||Hemolyzed or lipemic samples.|
|Processing notes||Deliver tube to Hematology asap for processing. Freeze serum at -20C. Order Quest test #14627.|
|Units||% release at 3 doses of UFH (0.1 IU/mL, 0.35 IU/mL and 100 IU/mL)|
|Critical value||Quest Priority-2: ≥ 20 % release|
|Synonyms||HIT; heparin induced thrombocytopenia; SRA; unfractionated heparin|
|Turn around times||Test performed once a week. Turnaround time: 10-14 days.|
|Additional information||A negative test does not exclude thrombotic risk due to heparin induced thrombocytopenia (HIT), nor does a positive test exclude other causes of thrombocytopenia. Correlation with heparin-PF4 complex antibody test, platelet counts, and clinical finding can be important for diagnosis of HIT.
A Heparin-PF4 Complex Antibody test (HPA) should be performed prior to requesting a Serotonin Release Assay (SRA). If an SRA is requested prior to the result of HPA testing being completed, the following text will be sent: "Test not performed. If clinically indicated, after results of heparin-platelet factor 4 antibody test (HPA) are available, please re-order SRA. It is generally recommended that patient not receive heparin for 24 hours prior to SRA testing."
The SRA is often not necessary in the presence of a positive test for heparin-PF4 antibodies.
|Last Updated||4/14/2011 2:43:18 PM|