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Lab Manual for Moffitt-Long and Mount Zion

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Reptilase Time

Item Value
Approval req'd? No
Available Stat? No
Test code REPT
Performed by Quest
Sendout? Yes
Method Clot based assay
Collection Instructions 1. Check the expiration date on the label of the blue top vacutainer before drawing the patient.

2. For blood collection in a sodium citrate blue top, the tube must be filled to above the Minimum Fill Indicator on the tube. It is crucial to wait and allow the tube to stop filling before removing it from the needle.

3. With use of a butterfly needle, draw about 1 cc using a separate blue top to remove air from tubing, discard the first tube and then draw a second blue top tube filled to the full extent of the vacuum.

4. Tubes should not be filled past the Maximum Fill dashed line by either using a syringe or removing the tube cap.

For patients with Hct's ≥ 55% please contact Hematology (x3-1747) to obtain blue top tubes with adjusted citrate volumes in order to maintain the proper citrate to plasma ratio for coagulation studies.
Container type Blue top filled to full extent of vacuum
Amount to Collect 2.7 mL blood
Sample type Citrated plasma
Preferred volume 1 mL plasma
Min. Volume 0.5 mL plasma
UCSF Rejection Criteria Samples collected in outdated blue top vacutainer. Over-filled or under-filled tubes may be rejected
Processing notes Specimen to be delivered to hematology lab for centrifugation and freezing prior to send out. Transport frozen on dry ice to China Basin. Do not allow the specimen to thaw. Order Quest # 37700X
Ref Lab Rejection Criteria Received refrigerated or room temperature.
Units seconds
Normal range 15-19 seconds
Turn around times Test run Wednesday Turnaround 1-8 days
Additional information A normal reptilase time in the setting of a prolonged thrombin time indicates the presence of an inhibitor of thrombin. Unlike thethrombin time, the Reptilase Clotting Time is not prolonged by use of heparin
or hirudin.

The reptilase time can also be used as a screening test for dysfibrinogenemia. Confirmatory testing for dysfibrinogen is by comparison of functional and immunologic fibrinogen levels.
CPT coding 85635-90
LOINC code 6683-7
Last Updated 10/2/2010 11:52:31 AM
Entry Number 818
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