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Lab Manual for Moffitt-Long and Mount Zion

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Pyruvate, plasma
Important: Read Collection Instructions BEFORE obtaining samples.

Item Value
Approval req'd? No
Available Stat? No
Test code PYRU
Test group Pyruvate
Performed by Quest
Sendout? Yes
Method Enzymatic
Patient Preparation An 8 hour fast before specimen collection is preferred.
Collection Instructions Note: If the patient cannot tolerate the 2 mL preferred volume, contact the laboratory at x3-1667 and ask to speak to a Processing or Chemistry supervisor. The volume of blood that can be collected (minimum 1.0 mL) needs to be coordinated with the lab so we can create a special perchloric acid tube containing an equal volume of Perchloric acid for use in stabilizing the sample.

Whole blood:

1. Collection kits containing a conical tube with 2 mL 7% Perchloric acid and 2 mL Lavender top (EDTA) vacutainers are available from the 5th Floor laboratory and the ACC phlebotomy area. Samples must be collected with this kit.

2. Place the tube containing 2 mL Perchloric acid in a cup of ice to chill

3. Draw 2 mL blood into a Lavender top (EDTA) vacutainer with minimum stasis (be sure the container fills completely) The second lavender top tube in the kit is for back-up purposes only.

4. Gently mix the EDTA sample by inversion 4-6 times

5. Carefully open the Perchloric acid tube (avoid skin contact with the Perchloric acid)

6. Using a gauze or safety wipe carefully remove the cap from the vacutainer to avoid creating droplets

7. Pour the entire contents of the vacutainer into the Perchloric acid tube

8. TIGHTLY cap the tube containing the blood/Perchloric acid mixture and mix vigorously by hand.

9. Attach a patient label to the conical tube containing the mixture

9. Place the tube back into the cup of ice and hand carry immediately to the lab for processing.

Click here for a printable version of these instructions
Container type Lavender top (on ice)
Amount to Collect 2 mL blood (be sure to fill vacuatiner completely)

Note: If the patient cannot tolerate this volume contact the laboratory at x3-1667 and ask to speak to a Processing or Chemistry supervisor. The volume of blood that can be collected (minimun 1.0 mL) needs to be coordinated with the lab so we can create a speacial perchloric acid tube containing an equal volume of Perchloric acid for use in stabilizing the sample.
Sample type Plasma filtrate
Preferred volume 2.5 mL plasma filtrate
Min. Volume 0.6 mL plasma filtrate
UCSF Rejection Criteria Sample not mixed with Perchloric acid within 30-60 seconds after collection. Not delivered on ice.
Processing notes Provide sample to Chemistry staff immediately upon receipt.

Chemistry will centrifuge the chilled tube containing EDTA whole blood and 7% Perchloric acid for 10 minutes at 3000 rpm. Transfer the supernatant into a specimen vial and label with a Sunquest label. Record the approximate volume of the supernatant on the requisition. Freeze at -20C.

Store and ship the sample frozen. Order Quest # 765Z.
Units mg/dL
Normal range 0.30 - 1.50 mg/dL
Synonyms Pyruvic acid
Stability Refrigerated 1 month, frozen at -20C 3 months
Turn around times Test run Monday-Friday. Turnaround time: 1-4 days.
Additional information To convert mmol/L (SI units) to conventional mg/dL, multiply by 8.77.
CPT coding 84210-90
LOINC code 14121-8
Last Updated 5/15/2013 11:35:58 AM
Entry Number 803
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