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|Method||Fixed time nephelometry|
|Patient Preparation||An 8 hour fast before specimen collection is required.|
|Collection Instructions||1. Check the expiration date on the label of the blue top vacutainer before drawing the patient.
2. For blood collection in a sodium citrate blue top, the tube must be filled to above the Minimum Fill Indicator on the tube. It is crucial to wait and allow the tube to stop filling before removing it from the needle.
3. With use of a butterfly needle, draw about 1 cc using a separate blue top to remove air from tubing, discard the first tube and then draw a second blue top tube filled to the full extent of the vacuum.
4. Tubes should not be filled past the Maximum Fill dashed line by either using a syringe or removing the tube cap.
|Container type||Blue top filled to full extent of vacuum, Lavender top|
|Amount to Collect||2.7 mL blood|
|Sample type||Citrated plasma (EDTA plasma acceptable)|
|Preferred volume||1 mL plasma|
|Min. Volume||0.5 mL plasma|
|UCSF Rejection Criteria||Samples collected in outdated blue top vacutainer. Over-filled or under-filled tubes may be rejected|
|Processing notes||Centrifuge within one hour of blood draw at 3200 rpm for 15 min. Remove plasma using plastic pipet and freeze immediately at -20C. Order Quest # 59915P|
|Normal range||8-14 mg/dL|
|Turn around times||Test run Tuesday and Friday mornings. Turnaround: 3-7 days.|
|Last Updated||6/21/2016 12:17:49|