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Red Cell Osmotic Fragility
|Performed by||Oakland Children's Hospital|
|Collection Instructions||By appointment only, contact Hematology at x3-1747. Collect samples on Monday-Thursday only and transport on ice to Hematology (M524) before 1000 hours for immediate processing.
As this specific assay is not available through LabCorp, it is available to B&T patients only if the ordering physician provides prior authorization from B&T.
|Container type||Yellow top (ACD) (on ice) AND Lavender top (on ice)|
|Amount to Collect||8.5 mL ACD blood (Yellow top)
2 mL EDTA blood (Lavender top)
|Sample type||EDTA AND ACD anticoagulated whole blood|
|Preferred volume||ACD 8.5 mL blood AND Lavender 2 mL blood|
|Min. Volume||ACD 2 mL blood AND Lavender 1 mL blood|
|UCSF Rejection Criteria||Not delivered on ice or received after 1000 hours Monday-Friday|
|Processing notes||Order RET and CBC if there is no CBC or CBCD on the same sample. Arrange refrigerated shipment Monday-Thursday only.
Wrap sample in paper towels to prevent freezing and ship on wet ice to Oakland Childrens Hospital via MCI courier.
If not received on ice, the specimen must be
accompanied by a travel control sample to verify the adequacy of transportation conditions or will be refused as unsuitable for assay.
|Normal range||See Additional Information|
|Stability||Refrigerated samples are stable for only 24 hours|
|Turn around times||Test run Monday-Friday only. Turnaround time: usually 2-4 days after sample is received.|
|Additional information||This test, which is performed by the Special Hematology Laboratory of Children's Hospital of Oakland, replaces the classical RBC Osmotic Fragility test, and requires a Reticulocyte Count as well as a CBC for interpretation. This test is not recommended for children < 6 months old. Cells are tested over
a gradient from 100-500 mosmol/kg.
|Last Updated||12/19/2012 4:31:13 PM|
If you have additional questions regarding this test, please call: 415-353-1667