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Lab Manual for Moffitt-Long and Mount Zion

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Onchocerca volvulus

Item Value
Approval req'd? No
Available Stat? No
Test code P412
Performed by Microbiology
In House Availability Monday-Friday, day shift
Method Wet mount microscopy
Collection Instructions Contact Microbiology x3-1268 to obtain special multi-well container for collecting and transporting samples. The specimens should be taken from an affected area or from scapular, gluteal and calf areas in the African form of disease, from the scapular, deltoid and gluteal areas in the Central American form of the disease.

Submit Friday samples before 1230 hours.
Container type Clean container, urine cup, Lavender top
Amount to Collect See preferred volume
Sample type Skin, Random urine, EDTA whole blood
Preferred volume 6 skin snips 2-3 mm in diameter obtained with a corneoscleral punch or by lifting the skin with a needle point and excising epidermis and dermis with a razor
UCSF Rejection Criteria Improperly collected or submitted samples
Normal range
Stability Skin snip at room temperature: 24 hours
Refrigerated urine: 24 hours
Turn around times 1-3 days
Additional information If a wet mount is positive, B409 (Giemsa stain) will be ordered at an additional charge to differentiate Onchocerca from Loa loa.

Urine (see Parasites-Urine ) or peripheral blood (see Filaria) may sometimes show the parasite in heavily infected individuals.
LOINC code 33017-5
Last Updated 3/24/2013 8:05:36 PM
Entry Number 693
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