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Neutrophil Cytoplasmic Antibodies

Item Value
Approval req'd? No
Available Stat? No
Test code ANCA
Test group Neutrophil Antibodies
Performed by Immunology
In House Availability Tuesday (day shift)
Method Chemiluminescence
Collection Instructions Avoid hemolysis
Container type Gold top
Amount to Collect 2 mL blood
Sample type Serum
Preferred volume 1 mL serum
Min. Volume 0.5 mL serum
UCSF Rejection Criteria Grossly Hemolyzed, Lipemic or Icteric
Processing notes Freeze serum at -20°C.
Units Chemiluminescence units (CU)
Normal range Myeloperoxidase Antibody (MPO) and Proteinase-3 Antibody (PR3)
Negative < 20.0 CU
Positive ≥ 20.0 CU
Synonyms ANCA; Anti-Neutrophil Antibodies; anti-MPO; Myeloperoxidase antibodies; anti-myeloperoxidase; Proteinase-3 Antibody; anti-Proteinase-3 Antibody; PR3; anti-PR3;
Turn around times 2-8 days
Additional information This test may be useful in discriminating among the various vasculitides. Cytoplasmic (c-ANCA) antibodies giving diffuse cytoplasmic staining are directed against Proteinase-3, and are suggestive of a spectrum of diseases which includes Wegener's Granulomatosis, polyarteritis nodosa, Churg-Strauss syndrome, and primary necrotizing and crescentic glomerulonephritis. Perinuclear (p-ANCA) staining is due to antibodies to myeloperoxidase (MPO) and other antigens such as elastase and lactoferrin, and is less specific.
CPT coding 83516 X 2
LOINC code 45151-8
Last Updated 5/28/2016 6:13:03 PM
Entry Number 684
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