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Neonatal Alloimmune Thrombocytopenia-Fetal evaluation
|Approval req'd?||REQUIRES INSURANCE INFORMATION, AUTHORIZATION FROM HEALTH CARE PROVIDER FOR TEST AND PRIOR APPROVAL BEFORE COLLECTION OF SAMPLES.
Contact Blood Bank at x3-1313 to coordinate testing. Blood bank will complete the Platelet & Neutrophil Immunology Lab requisition and order the appropriate test code. The test will be entered as a Miscellaneous Outside Laboratory Test (MOLT).
|Test group||Neonatal Alloimmune Thrombocytopenia|
|Performed by||Blood Center of Wisconsin|
|Method||PCR, Luminex bead methodology|
|Collection Instructions||See information under Approval req'd BEFORE collecting sample
Discard first 2 mL of fluid. Collect 30 mLs Amniotic Fluid. Sample is to be collected in two 15 mL. orange screw top polypropylene tubes (less if early amniocentesis). If the sample is grossly bloody, the results may be inconclusive and a repeat sample will be requested.
Complete and submit Blood Center of Wisconsin Platelet Immunology Lab form and send with the samples.
|Container type||Orange top polypropylene tubes x2
Lavender top tubes x2 (from mother)
|Amount to Collect||30 mL Amniotic fluid|
|Sample type||Amniotic fluid|
|Preferred volume||30 mL Amniotic fluid|
|Min. Volume||10 mL Amniotic fluid|
|Processing notes||Keep samples at room temperature. DO NOT CENTRIFUGE for any reason.
Send all tubes and completed paperwork ASAP to the Cytogenetics Laboratory at China Basin.
Cytogenetics Laboratory will expand amniocytes to two T25 flasks and arrange shipping to The Blood Center of Wisconsin through the send out department at China Basin. A minimum of 5 x 10e6 cultured amniotic cells is required.
Cultured cells: Media should be added to the flasks by Cytogenetics, sealed to avoid leakage and shipped at room temperature.
Package specimens and ship at room temperature by Federal Express Monday - Thursday only.
All samples should reach BCW within 24 hours of addition of media and no later than 1 pm on Friday. Mark box with Keep at Room Temperature and UP arrows to indicate the box must be maintained upright during shipment. Mark OVERNIGHT on Federal Express form.
Shipping Address: Client Services/PNIL, Blood Center of Wisconsin, 638 North 18th St., Milwaukee, WI 53233-2121
Phone: 800 245-3117, ext 6250
PLEASE CALL THE LABORATORY (800-245-3117, ext 6255) FOR ADVICE IF YOU WILL SHIP SAMPLES NEAR A MAJOR HOLIDAY.
|Synonyms||Fetal platelet antigen genotyping; NAIT; anti-platelet antibodies; platelet specific antibodies; PlA1; Bak; Pen; NAT; NATP|
|Stability||24 hrs from time media is added to cell culture.|
|Turn around times||1 week|
|CPT coding||83891-90, 83896-90 x18, 83900-90 ,83901-90 x5, 83912-90 for Genotyping Panel.
83891-90, 83896-90 x2, 83898, 83912-90 - per system for Individual Antigen Systems.
|Last Updated||3/24/2013 7:02:03 PM|
If you have additional questions regarding this test, please call: 415-353-1667