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|Method||Colorimetry w/synthetic chromagenic ubstrate|
|Collection Instructions||1. Check the expiration date on the label of the blue top vacutainer before drawing the patient.
2. For blood collection in a sodium citrate blue top, the tube must be filled to above the Minimum Fill Indicator on the tube. It is crucial to wait and allow the tube to stop filling before removing it from the needle.
3. With use of a butterfly needle, draw about 1 cc using a separate blue top to remove air from tubing, discard the first tube and then draw a second blue top tube filled to the full extent of the vacuum.
4. Tubes should not be filled past the Maximum Fill dashed line by either using a syringe or removing the tube cap.
For patients with Hct's ≥ 55% please contact Hematology (x3-1747) to obtain blue top tubes with adjusted citrate volumes in order to maintain the proper citrate to plasma ratio for coagulation studies.
|Container type||Blue top filled to full extent of vacuum|
|Amount to Collect||2.7 mL blood|
|Sample type||Citrated Plasma|
|Preferred volume||1 mL plasma|
|Min. Volume||0.5 mL plasma|
|UCSF Rejection Criteria||Samples collected in outdated blue top vacutainer. Over-filled or under-filled tubes may be rejected|
|Processing notes||Separate plasma by centrifugation, then recentrifuge the separated plasma to avoid spurious results from platelet contamination. Promptly freeze an 0.5 mL aliquot of the respun plasma in a plastic vial at -70C. Call MCS for pick-up and ship on dry ice. The sample must be accompanied by a completed Mayo Medical Laboratories "Coagulation Request Form". Order MAYO# 9084|
30-36 wk premature infants may not reach
adult levels for up to 90 days of age
|Synonyms||Alpha-2-Antiplasmin; A2-AP; Alpha-2-plasmin inhibitor; A2-antiplasmin; A2-PI|
|Turn around times||7 days.|
|Additional information||Antiplasmin Activity is primarily due to alpha-2-antiplasmin, w/ some interference from alpha-2-macroglobulin.|
|Last Updated||9/18/2012 2:17:26 PM|