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Lung Biopsy and Bronchoalveolar Lavage
|In House Availability||Set up daily, day and evening shifts|
|Collection Instructions||Hand carry these specimens to the laboratory, L 515, to promote rapid processing and to ensure communication of priorities if only a limited amount of sample is available. At hours when Microbiology is closed, bring specimens to Specimen Receiving at the main laboratory in Moffitt.
Provide all of the clinical information requested when submitting the Microbiology requisition, and specifically indicate whether the patient has recently undergone organ transplantation or is otherwise immunocompromised.
See Notes for additional information
|Container type||Clean container|
|Amount to Collect||See preferred volume|
|Sample type||BAL, Tissue|
|Preferred volume||Sample Requirements:
Bronchial lavage fluid: 30 mL of fluid in a sputum (Lukens) trap
Transbronchial lung biopsy tissue (particularly suitable for possible infection with fungi, mycobacteria or Legionella): Submit in a sterile black top tube or a Red top sterile vacutainer in non-bacteriostatic saline or-if Legionella is suspected-in phosphate buffer or sterile water (saline is toxic to Legionella species).
Open lung biopsy: In a sterile container, submit at least 3 cm3 or a 1/2-1 inch cube of tissue. The specimen should be wrapped in gauze moistened with non-bacteriostatic saline to prevent dessication. Saline is toxic to Legionella, and if these species are a diagnostic concern phosphate buffer should be employed.
Phosphate buffer is available in refrigerator #10 at Specimen Receiving in the main laboratory.
|Processing notes||Maintain sample at room temperature|
|Stability||BAL: Refrigerated 24 hours
Biopsy: Room temperature 24 hours
|Additional information|| Bronchial lavage fluid from immunocompromised patients is routinely processed as noted below; for all other specimens you must indicate which of the following tests are desired. If there is any doubt regarding the adequacy of the volume of sample submitted, specify the priority in which the tests should be performed.
Recommended evaluation of Specimens from Immunocompromised Patients
Tissue obtained for histologic examination should be submitted directly to Surgical Pathology, M 580, x31633, open 0730-1800 hours Monday-Friday. At other hours, obtain the telephone number of the resident on call from the page operator.
Bronchial lavage fluid is examined for Pneumocystis carinii by Cytology. M 545, x31613, open 0800-1500 hours, Monday-Friday. Upon request, specimens received before 1000 hours will be reported out the same day.
|Last Updated||4/28/2010 4:00:12 PM|
If you have additional questions regarding this test, please call: 415-353-1667