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Leukemia/Lymphoma Markers

Item Value
Approval req'd? No
Available Stat? No
Test code
LEUM Blood
LEUMBF Bone marrow and other body fluids
Test group CD
Performed by Immunology
In House Availability Monday-Saturday (day shift)
Method Flow cytometry
Collection Instructions Bone marrow aspirates should be held at room temperature. Fine needle aspirates should be refrigerated until they can be assayed (this medium is available in refrigerator at Specimen Receiving in the main laboratory).

Test performed Monday-Friday at 1200, results available at 1700 hours the following day. Saturday at 1200, results available at 1700 hours on Monday.

If results are required for immediate patient treatment, for which the above schedule will not suffice, contact the Laboratory Medicine resident.
Container type
Blood or marrow Lavender top
CSF CSF tube or sterile collection tube
Body Fluid 50 mL conical bottom plastic tube with blue screw cap preferred
Amount to Collect 3 mL blood
Sample type EDTA whole blood, Bone marrow, Unfixed tissue, Tissue aspirates, CSF, Body fluids (with approval)
Preferred volume Amount of specimen needed varies call Immunology, x3-1712, for consultation

For body fluids 80-100 mL is generally sufficient but this is dependent on the cell count.
UCSF Rejection Criteria Reject samples that have been frozen, stored in fixative, transported or stored at improper temperatures.
Processing notes Hold bone marrow specimens at room temperature, but refrigerate fine needle aspirates in special holding medium. Do NOT centrifuge. Each specimen should be assigned its own accession number.

If specimens are delivered after 1200 hours on Saturday, anytime Sunday or on a holiday contact the resident on call.
Normal range See Additional Information
Synonyms AML; acute myeloid leukemia; ALL; Acute lymphocytic leukemia; Acute lymphoblastic leukemia; T-ALL; B-ALL; CLL; Chronic lymphocytic leukemia; Mantel cell lymphoma; Immunoblastic lymphoma; small cell lymphoma; follicular lymphoma; large cell lymphoma; Burkitt's lymphoma; diffuse lymphoma, small cleaved cell lymphoma; lymphoblastic lymphoma; flow cytometry; Sezary Cells; Sezary Syndrome; Mycosis fungoides
Turn around times Preliminary result available from laboratory in 1-2 days. Written interpretive report sent within 7 days.
Additional information An interpretation of this test by a laboratory physician will automatically be performed and billed for separately.

This testing is appropriate for the evaluation of suspected hematologic malignancies, including Sezary's syndrome. Groups of monoclonal antibodies selected as appropriate for the diagnoses under consideration, are employed to detect one or more of the following phenotypic subsets:

Antigen Cells Detected/Comments
CD2 T cells (E rosette sheep RBC receptor)
CD3 T cells
CD4 helper and inducer T cells and monocytes
CD5 T cells, NK cells and B cell subsets, most CLL
CD7 T cells, lost on most sezary cells
CD8 suppressor and cytotoxic T cells and NK cells
CD10 immature lymphoid and germinal center cells, many pre-B ALL
CD11c monocytes, NK cells and B cell subsets
CD13 myeloid lineage cells
CD14 monocytes
CD15 myeloid and Reed-Sternberg cells and rare T cells
CD16 NK cells and T cell subsets
CD19 mature B and pre-B cells
CD20 mature B cells, some T cells
CD22 mature B cells
CD23 B cells, most CLL
CD25 activated T and B cells
CD33 myeloid lineage cells
CD34 hematopoietic progenitor (stem) cells
CD38 pre-B, pre-T, activated T and plasma cells
CD45 all leukocytes
CD52 CAMPATH-1, lymphoid malignancies
CD56 NK cells and T cell subsets
CD61 megakaryocytes and platelets
CD117 c-kit, myeloid lineage
Kappa kappa light chain-positive B cells
Lambda lambda light chain-positive B cells

This test was developed and its performance characteristics determined by the Clinical Laboratories at the Medical Center at UC San Francisco. Only some of the reagents used have been cleared or approved by the U.S. Food and Drug Administration.

An interpretation of this test by a laboratory physician will automatically be performed and billed for separately.
CPT coding 88184; 88185 x 21
LOINC code 54226-6
LDT or Mod FDA? Yes
Last Updated 6/22/2016 12:51:35
Entry Number 615
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