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Lab Manual for Moffitt-Long and Mount Zion

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CD3 dose estimation

Item Value
Approval req'd? No
Available Stat? No
Test code CD3D
Test group CD
Performed by BMT Lab
In House Availability Test performed Monday-Friday at 0800, 1300, and 1500 hours.
Method Flow cytometry
Collection Instructions Specimens must reach the main laboratory a minimum of 30 minutes prior to the next scheduled run.

Testing on Saturday requires prior arrangement with HPCT (call x31789)
Container type Lavender top for blood or ACD for pheresis sample.
Amount to Collect 1 mL blood
Sample type EDTA whole blood or Apheresis sample
Preferred volume 1 mL blood
Min. Volume 0.5 mL blood
Processing notes Notify HPCT (x31789) when a specimen arrives at Central Processing. Samples are prepared from leukopheresis and must be delivered to Immunology before 1200 hours.
Normal range Not applicable
Synonyms CD3% for dosing calculations; flow cytometry
Turn around times 3 hours after receipt of sample or next day.
Additional information This test can be ordered with the CD34 assay and performed on the same sample.

Immunology will report the percentage of the total WBCs which are CD3 cells. From the leukopheresis volume, the total leukocyte count in the pheresis, and the patient's weight, the Blood Bank will calculate the absolute number of CD3-positive cells (T lymphocytes) per kg to be infused into the patient.

This test was developed and its performance characteristics determined by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. Food and Drug Administration.
LOINC code 8122-4
LDT or Mod FDA? Yes
Last Updated 2/25/2013 9:20:10 AM
Entry Number 611

If you have additional questions regarding this test, please call: 415-353-1667

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