UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources

Click here for more information about laboratory operations and procedures

If you have additional questions regarding this test, please call: 415-353-1667

Search Our Test Menu

Lymphocyte subsets

Item Value
Approval req'd? No
Available Stat? No
Test code TBHSD (Blood) (with CBC & diff)
TBHSBF (body fluid)
Test group CD
Performed by Immunology
In House Availability Monday-Saturday (day shift)
Method Flow cytometry
Container type Lavender top
Amount to Collect 3 mL blood
Sample type EDTA whole blood
Preferred volume 3 mL blood
UCSF Rejection Criteria Refrigerated sample received. Sample > 48 hours old when received.
Processing notes DO NOT refrigerate, store at room temperature and ship to China Basin.

Order CBCD if not already ordered on the same sample.
Units % and x106 cells/L
Normal range
Subset Percentage Absolute
CD3 (T) Cells 55-88% 715-2431 x106 cells/L
CD4 (helper-inducer) T Cells 24-64% 440-1496 x106 cells/L
CD8 (cytotoxic-suppressor) T Cells 14-40% 270-918 x106 cells/L
CD19 (B) Cells 6-22% 140-476 x106 cells/L
CD56 Natural Killer (NK) cells 3-29% 160-544 x106 cells/L

CD4/CD8 (H/S) Ratio: 0.7-3.9

Note: Reference values are for ≥ 18 year olds. For pediatric ranges please see:

Kotylo, PA, et al. 1993. Reference ranges for Lymphocyte Subsets in Pediatric Patients. Am. J. Clin. Pathol 100:111-115

Lin, S-C., et al. 1998. Age-Related Changes in Blood Lymphocytes of Chinese Children. Ped. Allergy Immunol. 9:215-220

Melaranci, C., et al. 1992. T. Cell Subpopulations in Pediatric Healthy Children: age-normal values. J. Clin. Lab. Immunol. 30:143-149
Synonyms CD3; CD4; CD19; CD56; T cells; B cells; NK cells; CD4/CD8 ratio; H/S ratio; helper/suppressor ratio; cytotoxic T cells; suppressor T cells; absolute T cell count; T cell markers; T subsets; B cell markers; T&B cells; T & B cells; T and B cells; T4/T8 ratio; flow cytometry
Turn around times 2-3 days
Additional information Absolute cell counts require a CBC w/Differential on the same sample, which will be ordered and charged separately if not otherwise available.

CD4 results are automatically forwarded to California public health per regulations. However, this does not absolve the ordering provider from any additional reporting if such is required.
CPT coding 86360, 86359, 86357, 86355, 85025
LOINC code 30364-4
Last Updated 10/14/2016 12:35:28 PM
Entry Number 610
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center