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Lymphocyte subsets

Item Value
Approval req'd? No
Available Stat? No
Test code TBHS
Test group CD
Performed by Immunology
In House Availability Monday-Saturday (day shift)
Method Flow cytometry
Container type Lavender top
Amount to Collect 3 mL blood
Sample type EDTA whole blood
Preferred volume 3 mL blood
UCSF Rejection Criteria Refrigerated sample received. Sample > 48 hours old when received.
Processing notes DO NOT refrigerate, store at room temperature and ship to China Basin.

Order CBCD if not already ordered on the same sample.
Units % and x106 cells/L
Normal range
Subset Percentage Absolute
CD3 (T) Cells 55-88% 715-2431 x106 cells/L
CD4 (helper-inducer) T Cells 24-64% 440-1496 x106 cells/L
CD8 (cytotoxic-suppressor) T Cells 14-40% 270-918 x106 cells/L
CD19 (B) Cells 6-22% 140-476 x106 cells/L
CD16, CD56 Natural Killer (NK) cells 3-29% 160-544 x106 cells/L

CD4/CD8 (H/S) Ratio: 0.7-3.9

Note: Reference values are for ≥ 18 year olds. For pediatric ranges please see:

Kotylo, PA, et al. 1993. Reference ranges for Lymphocyte Subsets in Pediatric Patients. Am. J. Clin. Pathol 100:111-115

Lin, S-C., et al. 1998. Age-Related Changes in Blood Lymphocytes of Chinese Children. Ped. Allergy Immunol. 9:215-220

Melaranci, C., et al. 1992. T. Cell Subpopulations in Pediatric Healthy Children: age-normal values. J. Clin. Lab. Immunol. 30:143-149
Synonyms CD3; CD4; CD19; CD56; T cells; B cells; NK cells; CD4/CD8 ratio; H/S ratio; helper/suppressor ratio; cytotoxic T cells; suppressor T cells; absolute T cell count; T cell markers; T subsets; B cell markers; T&B cells; T & B cells; T and B cells; T4/T8 ratio; flow cytometry
Turn around times 2-3 days
Additional information Absolute cell counts require a CBC w/Differential on the same sample, which will be ordered and charged separately if not otherwise available.

CD4 results are automatically forwarded to California public health per regulations. However, this does not absolve the ordering provider from any additional reporting if such is required.
LOINC code 30364-4
Last Updated 8/18/2014 9:12:09 AM
Entry Number 610
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