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Lab Manual for Moffitt-Long and Mount Zion

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Leukocyte Phenotyping

Item Value
Approval req'd? No
Available Stat? No
Test code See individual tests in 'Additional information'
Test group CD
Performed by Immunology
Method Flow cytometry
Collection Instructions All assays use live cells, and specimen collection and handling instructions must be closely followed.
Container type Lavender top
Amount to Collect 3 mL blood
Sample type EDTA whole blood
Processing notes Keep sample at room temperature
Normal range
Synonyms Flow cytometry; Leukocyte antigens; CD; CALLA; CD3; CD4; CD19; CD56; T cells; B cells; NK cells; CD4/CD8 ratio; H/S ratio; helper/suppressor ratio; cytotoxic T cells; suppressor T cells; absolute T cell count; T cell markers; T subsets; B cell markers; T&B cells; T & B cells; Tand B cells; T4/T8 ratio; leukemia; lymphoma; ALL; AML
Stability Room temperature 1 day
Additional information Numerous subpopulations of leukocytes, especially lymphocytes, can be identified and enumerated. The cells are identified by their surface antigen using clusters of differentiation (CD) nomenclature.

Results are usually expressed as percent of lymphocytes; to obtain the absolute number of cells, a CBC w/Differential count must be ordered and separately charged.

The assays routinely offered by the Immunology section of the Clinical Laboratories, in alphabetic order, are:

CD3, CD19 and CD 16, CD 56 (T, B and NK) cells,with CD4 and CD8 Subsets and CD4/CD8 Ratio:
CD3 (total T) cells and the CD4 (helper-inducer T) and CD8 (cytotoxic-suppressor T) subsets are determined, in addition to CD19 (total B) cells and CD 16, CD56 (NK) cells. Although this test could be used in HIV infections to monitor CD4 T cells, it is more useful in situations such as congenital immunodeficiencies. Click here for information

CD4 T and CD3 (Helper-Inducer and Total T) Cells:
This test is most often used to determine eligibility for anti-retroviral therapy and to follow HIV infection. Includes directly measured counts of CD3 (Total T), CD4 T cells, CD8 T cells and a calculated CD4/CD8 ratio are provided. Click here for information

CD34 Stem Cells:
These cells can be measured in peripheral blood or a leukopheresis collection and the volume of stored cells which must be infused into the patient for a greater likelihood of a successful autologous graft more accurately estimated. Click here for information

Leukemia/Lymphoma Markers:
A series of antibodies, to myeloid as well as lymphoid cells, are employed to characterize leukemias and/or lymphomas. This test is used AFTER the diagnosis of a neoplasm has been made and when the determination of the cell type is in question; it CANNOT be used by itself to diagnose malignancy. Click here for information

Single and/or multiple Subsets:
Phenotyping for single subsets other than CD4 is most often used in evaluating immunodeficiences or in monitoring antileukocyte therapy. Possible subsets include CD3 (T), CD19 (B), CD20 (B), CD33 (myeloid), CD52 (lympoid cells). To request subset typing, contact Immunology, x3-1712.

An interpretation of this test by a laboratory physician will automatically be performed and billed for separately.
LDT or Mod FDA? Yes
Last Updated 2/24/2014 9:41:57 AM
Entry Number 609

If you have additional questions regarding this test, please call: 415-353-1667

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