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Lab Manual for Moffitt-Long and Mount Zion

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Chromosome analysis, Adult Leukemia

Item Value
Approval req'd? Yes, contact Cytogenetics at x3-4813 if requested on blood samples.
Available Stat? No
Test code CYTLL
Performed by Medical Genomics - Cytogenetics
Method Giemsa banding and brightfield microscopy
Collection Instructions Bone marrow is the preferred specimen, but heparinized peripheral blood may be submitted if a large number of malignant cells are present.

Collect bone marrow in a syringe, transfer to Dark Green top vacutainer and gently invert the tube several times for good mixing.

If a dry tap is obtained, consult the Laboratory Medicine resident in Hematology regarding the possible submission of a green top tube of peripheral blood. Contact Hematology if the specimen is more than 24 hours old.

Complete and submit a "Neoplastic Cytogenetics" form and send with the samples.

Click here for Neoplastic Cytogenetics Requisition

Keep sample at room temperature.
Container type Blood & bone marrow aspirate: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844. Dark Green top also acceptable

Bone marrow core: 15 mL centrifuge tube with transport media (RPMI, FBS, L-Glutamine, Sodium Heparin and PenStrep). Available from Cytogenetics, 415-353-3844.
Amount to Collect
Bone marrow 3 mL
Blood 3 mL
Bone core 2 cm
Sample type Heparinized bone marrow, whole blood or marrow core
Preferred volume
Bone marrow 3 mL
Blood 3 mL
Bone core 2 cm
Min. Volume
Bone marrow 2 mL
Blood 2 mL
Bone core 1 cm
UCSF Rejection Criteria Insufficient volume; unlabeled tubes; clotted samples; broken, leaking or contaminated tubes; frozen samples.
Processing notes Keep sample at room temperature. Do not centrifuge.
Normal range
Synonyms Cytogenetic analysis; leukemia cytogenetics; Karyotype; Karyotyping
Stability 48 hours
Turn around times 7-10 days
Additional information
CPT coding 88237 x2 and 88262
LOINC code 33893-9
Last Updated 1/22/2014 8:57:08 AM
Entry Number 607
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