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Leishmania Exam

Item Value
Approval req'd? No, but contact parasitologist in Microbiology (x31268) prior to collecting samples
Available Stat? No
Test code P403L
Test group Leishmania
Performed by Microbiology
In House Availability Monday-Thursday before 12:30 pm
Method Microscopy (Giema stain of smears)
Collection Instructions Contact parasitologist in Microbiology Lab x31268 prior to obtaining specimens, and to discuss appropriate types of samples and collection instructions.

Deliver the specimen to the laboratory within 30 minutes of collection.

Include travel/residence history on requisition.
Container type
Biopsy/tissue Sterile container
Lesion aspirate: Sterile container or syringe capped with Luer plug
Bone marrow: Lavender top (EDTA)
Amount to Collect
Tissue/biopsy 4 mm punch biopsies x 1-2
Lesion aspirate or bone marrow 0.5 mL
Sample type
Cutaneous/mucocutanous leishmaniasis Tissue/biopsy, lesion aspirate
Visceral leishmaniasis bone marrow
Preferred volume
Tissue/biopsy 4 mm punch biopsies x 1-2
Lesion aspirate or bone marrow 0.5 mL
Min. Volume
Tissue/biopsy 4 mm punch biopsies x 1-2
Lesion aspirate or bone marrow 0.5 mL
UCSF Rejection Criteria Fixed specimens
Normal range No Leishmania seen or detected
Synonyms Kala azar; Cutaneous leishmaniasis; Uta; Visceral leishmaniasis
Stability Room temperature 24 hours
Turn around times 1-3 days
Additional information Lesion biopsies and aspirates can be examined by stain and/or culture in patients with cutaneous or mucocutaneous leishmaniasis. Biopsy of liver, spleen, or lymph node, or bone marrow can be examined by stain and/or culture in visceral leishmaniasis (Kala azar). A portion of any tissue sample should also be submitted to Pathology.

Physician may also request Microbiology to send sample to CDC for PCR and DNA sequencing, and culture
CPT coding 87207
Last Updated 8/18/2015 12:03:38 PM
Entry Number 603
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