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Lactate Dehydrogenase, Serum / Plasma
|Clinical Questions||Is my patient actively hemolyzing his/her own or transfused RBCs?|
|Performed by||Parnassus & Mt. Zion Chemistry|
|In House Availability||Test available 24 hours per day 7 days per week|
|Method||Spectrophotometric, kinetic (lactate/NADH)|
|Collection Instructions||Samples should be submitted to the laboratory for processing within 2 hours of collection|
|Container type||Gold top or Light Green top|
|Amount to Collect||1 mL blood Click here for Microdetermination info|
|Sample type||Serum or plasma|
|Preferred volume||0.5 mL serum or plasma|
|Min. Volume||0.2 mL serum or plasma Click here for Microdetermination info|
|Processing notes||Centrifuge and separate serum/plasma from cells. Do not freeze.|
1. Pediatric reference ranges for children <1 year adapted from Soldin, Steven J. Pediatric Reference Intervals (method 3), 6th edition, AACC Press, 2007.
2. Normal range for children 1 to less than 15 years old adapted from Beckman Coulter's "Pediatric Reference Range Guidelines for Synchron Systems" Bulletin 9345
3. Normal range for adults was determined by testing 271 male and female healthy blood donors at UCSF. Adult range used for pediatric patients >15 years.
|Stability||Serum stable 2 days at 2-8°C. Do not freeze. Certain LDH isoenzymes (LD-4 and LD-5) are more sensitive to cold. Specimens may have variable decreases in LD activity when stored at 2-8°C, depending on their isoenzyme composition.|
|Turn around times||4 hours|
|Additional information||Hemolysis may increase the LDH result.
Certain LDH isoenzymes (LDH-4 and LDH-5) are sensitive to cold exposure and cooling the sample may result in variable decreases in the LDH activity depending on the isoenzyme composition of the sample.
|Last Updated||4/18/2012 4:44:22 PM|
|Lab Procedure Link||Click here for Procedure|