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Lactate Dehydrogenase, Plasma / Serum

Item Value
Clinical Questions Is my patient actively hemolyzing his/her own or transfused RBCs?
Approval req'd? No
Available Stat? No
Test code LD
Test group LD
Performed by Parnassus, Mission Bay & Mt. Zion Chemistry
In House Availability Test available 24 hours per day 7 days per week
Method Spectrophotometric, kinetic (lactate/NADH)
Collection Instructions Samples should be submitted to the laboratory for processing within 2 hours of collection
Container type Light green top preferred, Gold top acceptable
Amount to Collect 1 mL blood
Click here for Microdetermination info
Sample type Plasma or serum
Preferred volume 0.5 mL plasma or serum
Min. Volume 0.2 mL plasma or serum
Click here for Microdetermination info
Processing notes Centrifuge and separate serum/plasma from cells. Do not freeze.
Units U/L
Normal range
Age Male & Female Male Female
< 1 month 125-735 U/L --- ---
1 month - 11 months 170-450 U/L --- ---
1 year - 4 years -- 140-304 U/L 142-297 U/L
5 years - 9 years -- 155-290 U/L 142-261 U/L
10 years - 14 years -- 115-257 U/L 122-234 U/L
≥ 15 years 102-199 U/L

1. Pediatric reference ranges for children <1 year adapted from Soldin, Steven J. Pediatric Reference Intervals (method 3), 6th edition, AACC Press, 2007.
2. Normal range for children 1 to less than 15 years old adapted from Beckman Coulter's "Pediatric Reference Range Guidelines for Synchron Systems" Bulletin 9345
3. Normal range for adults was determined by testing 271 male and female healthy blood donors at UCSF. Adult range used for pediatric patients >15 years.
Synonyms LD; LDH
Stability Serum stable 2 days at 2-8°C. Do not freeze. Certain LDH isoenzymes (LD-4 and LD-5) are more sensitive to cold. Specimens may have variable decreases in LD activity when stored at 2-8°C, depending on their isoenzyme composition.
Turn around times 4 hours
Additional information Hemolysis may increase the LDH result.

Certain LDH isoenzymes (LDH-4 and LDH-5) are sensitive to cold exposure and cooling the sample may result in variable decreases in the LDH activity depending on the isoenzyme composition of the sample.
CPT coding 83615
LOINC code 2532-0
Last Updated 7/24/2015 8:52:40 AM
Entry Number 589
Lab Procedure Link Click here for Procedure
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