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Insulin

Item Value
Test Update Information Effective March 15, 2011, Insulin testing is being performed in-house using the same instrument-methodology (Siemens Centaur) that was used by UC Davis Clinical Labs.
Approval req'd? No
Available Stat? No
Test code INS
Performed by China Basin Chemistry
Method Chemiluminescent Immunoassay
Patient Preparation An overnight fast is required prior to specimen collection or reference ranges will not apply.
Container type Gold top or Red top
Amount to Collect 2 mL blood
Sample type Serum
Preferred volume 1 mL serum
Min. Volume 0.6 mL serum
Processing notes Centrifuge promptly. Freeze serum at -20C.
Ref Lab Rejection Criteria Sample collected in other than Gold top or red top.
Units mU/L
Normal range
Fasting 3.3-22.1 mU/L


Reference range was adopted from UC Davis Clinical Labs based on in-house validation studies performed using the same instrument-methodology (Siemens Centaur) on samples from adult subjects with normal FBS (70-99 mg/dL) and A1c (4.3-5.6%).

Note: if patient was not fasting the normal ranges may not apply.

Pediatric reference ranges not available for this immunoassay platform.
Turn around times Test performed Thursday. Turnaround time: 3-10 days.
Additional information
Cross-reactivities of Insulin Analogs
Cross-reactivities, %
Analog and Concentration Access Advia Centaur Coat-A-Count E170 IMMULITE 2000
Lot 122 Lot 151
Insulin aspart
30 mIU/L 85.3 120 36.7 <0.7 14.7 9.3
100 mIU/L 80.0 124 57.0 <0.2 17.4 5.4
300 mIU/L 84.3 135 54.7 <0.07 40.7 8.0
1000 mIU/L 77.1 125 34.4 <0.02 40.7 13.0
Mean 81.7 126 45.7 28.4 8.9
Insulin glargine
30 mIU/L 91.7 129 32.0 <0.7 <6.7 8.3
100 mIU/L 85.0 140 46.0 <0.2 3.4 2.8
300 mIU/L 78.7 152 35.7 <0.07 11.1 1.6
1000 mIU/L 79.7 150 27.1 <0.02 13.2 1.8
Mean 83.8 143 35.2 9.2 3.6
Insulin lispro
30 mIU/L 78.7 86.7 37.0 <0.7 14.7 10.3
100 mIU/L 77.0 89.0 52.0 <0.2 18.0 6.3
300 mIU/L 79.3 92.3 49.7 <0.07 42.3 8.4
1000 mIU/L 80.2 89.2 33.4 <0.02 39.3 12.3
Mean 78.8 89.3 43.0 28.6 9.3
Table from: Owen WE, Roberts WL. Clin Chem. 2004 Jan;50(1):257-9

CPT coding 83525
LOINC code 20448-7
Last Updated 2/28/2017 10:06:32 PM
Entry Number 571
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