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Human Papilloma Virus High-Risk DNA Types
|In House Availability||Thursday (day shift)|
|Method||DNA:RNA hybrid capture by antibody with chemiluminescence|
|Collection Instructions||Place the cervical brush in the Cytyc ThinPrep PreservCyt solution.|
|Sample type||Cervical brush sample in preservative|
|Preferred volume||Sample in 10 mL preservative|
|Min. Volume||Sample in 6 mL preservative (insufficient for repeat testing)|
|UCSF Rejection Criteria||Specimen not received in required preservative|
|Processing notes||Store at room temperature until processed. Do not centrifuge the sample. Forward to Immunology at room temperature.|
|Normal range||Not detected|
|Turn around times||1-8 days|
|Additional information||Sufficient cellularity in the submitted sample is a requirement for accurate results. Low cellularity may produce false negative results.
Clinical utility, interferences, references, etc.: HPV DNA typing should be used in the management of women with aytpical squamous cells of undetermined significance. This test detects the HPV high-risk types. There are 13 gentypes/strains which are associated with HSIL and invasive cancer: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68. The assay does not differentiate among the HPV genotype/strains and is qualitative. Sensitivity and specificity vary depending upon the PAP smear result.
Rowe LR, Aldeen W, Bentz JS. 2004. Prevalence and typing of HPV DNA by hybrid capture II in women with ASCUS, ASC-H, LSIL and AGC on ThinPrep Pap tests. Diagn. Cytopathol. 30:426-432.
Derchain SF, Rabelo-Santos SH, Sarian LO, et al. 2004. Human papillomavirus DNA detection and histological findings in women referred for atypical glandular cells or adenocarcinoma in situ in their Pap smears. Gynecol. Oncol. 95:618-623.
Oliveira ER, Derchain SF, Rabelo-Santos SH, et al. 2004. Detection of high-risk human papillomavirus (HPV) DNA by Hybrid Capture II in women referred due to atypical glandular cells in the primary screening. Diagn. Cytopathol. 31:19-22.
Some low-risk types such as HPV6 and HPV42 cross react in this assay and high concentrations (> 4 ng/mL) of these low-risk types may produce a positive result in this assay.
|Last Updated||3/24/2013 7:11:28 PM|
If you have additional questions regarding this test, please call: 415-353-1667