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HIV Phenotyping

Item Value
Approval req'd? No
Available Stat? No
Test code MOLT (Order in Apex as 'Miscellaneous Outside Lab Test' using the complete test name above)
Test group HIV
Performed by Monogram Biosciences
Sendout? Yes
Method Culture
Collection Instructions Note: If both genotyping and phenotyping are requested for B&T patients, TWO separate tubes should be drawn.
Container type Lavender top
Amount to Collect 6 mL blood
Sample type EDTA plasma
Preferred volume 3 mL plasma
Min. Volume 3 mL plasma
Processing notes Centrifuge sample @ 100-1200g x 10-15 min. and separate plasma within 6 hours of sample collection. Transfer plasma to capped plastic tube and freeze at -20C. Do not thaw sample after freezing. Ship frozen with dry ice.

Note: If both genotyping and phenotyping are requested for B&T patients, aliquot the two tubes separately and freeze at -20C.
Normal range See Additional Information
Additional information HIV susceptibility testing (genotyping or phenotyping) should be reserved for patients who are: 1. Failing treatment requiring a change in regimen (prior to change in regimen) 2. Pregnant or 3. Have acute/recent HIV infection (w/in 3-6 months) in areas with high levels of transmission of resistance viruses (usually to AZT/3TC). Phenotyping requires a viral load > 500 copies/mL.

Reference: Hirsch MS et al. JAMA 2000, 283(10): 2442-4
Last Updated 2/20/2014 3:08:14 PM
Entry Number 514
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