UCSF Navigation Bar

UCSF Departments of Pathology & Laboratory Medicine Home Page

Lab Manual for UCSF Clinical Laboratories

Lab Manual for SFGH

Internal Resources

Click here for more information about laboratory operations and procedures

If you have additional questions regarding this test, please call: 415-353-1667

Search Our Test Menu

HIV-1 DNA, Qualitative, by PCR - for patients < 6 month of age

Item Value
Approval req'd? Yes, contact Immunology at x3-1712
Available Stat? No
Utilization Guidelines This test is generally performed ONLY on neonates and infants under 6 months of age. The sensitivity of the assay is 10 copies/mL whole blood and is only available as a qualitative assay. The test detects viral DNA that has integrated into the host genome within white blood cells rather than free RNA. In general, this test is considered interchangeable with HIV-1 RNA measured by PCR, but the minimum blood volume required is lower (1 mL whole blood vs. 1.1 mL plasma). The sensitivity of either test is low during the first month of life, and repeat testing at 1-2 months of age is recommended.
Test code MOLT (Order in Apex as 'Miscellaneous Outside Lab Test' using the complete test name above)
Test group HIV
Performed by Quest
Sendout? Yes
Method PCR
Container type Lavender top, Yellow (ACD) acceptable
Amount to Collect 3 mL blood
Sample type EDTA whole blood
Preferred volume 3 mL blood
Min. Volume 1 mL blood
Processing notes Do NOT centrifuge. Keep at room temperature. Complete form including specimen number, age, sex and date only; do not include patient name or hospital number. Order Quest # 98376P.
Normal range Negative
Synonyms Neonatal HIV screen
Turn around times Test performed Tuesday & Thursday. Turnaround time: 2-6 days.
Additional information The sensitivity of this assay is 10 copies of HIV-1 DNA per mL of whole blood.

This test is generally performed ONLY on neonates and infants under 6 months of age. The sensitivity of the assay is 10 copies/mL whole blood and is only available as a qualitative assay. The test detects viral DNA that has integrated into the host genome within white blood cells rather than free RNA. In general, this test is considered interchangeable with HIV-1 RNA measured by PCR, but the minimum blood volume required is lower (1 mL whole blood vs. 1.1 mL plasma). The sensitivity of either test is low during the first month of life, and repeat testing at 1-2 months of age is recommended.

Click here for the CDC definition of HIV infection in patients < 18 months of age
Medical Necessity? Yes. Click here for more information
LOINC code 44871-2
Last Updated 2/20/2014 3:07:42 PM
Entry Number 510
Lab Procedure Link href
UCSF home page UCSF home page About UCSF Search UCSF UCSF Medical Center