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Lab Manual for Moffitt-Long and Mount Zion

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Acanthamoeba & Naeglaria, Direct exam

Item Value
Approval req'd? No
Available Stat? Only the wet mount on CSF is offered stat
Test code P409
Test group Amoebic Meningitis
Performed by Microbiology
In House Availability Test performed Monday-Friday day shift
Collection Instructions Deliver specimen to laboratory within 30 minutes of collection. Do not refrigerate or freeze.
Container type CSF tube or sterile collection tube
Amount to Collect
CSF 5 mL
Tissue 6 mm
Sample type
Acanthamoeba CSF, brain biopsy, lung biopsy, corneal scraping or biopsy, skin abscess discharge, ear or nasopharyngeal discharge
Naeglaria CSF, brain biopsy
Preferred volume
CSF 5 mL
Tissue 6 mm
Min. Volume
CSF 2 mL
Tissue 3 mm
Processing notes Do not refrigerate or freeze sample. Transport asap to Microbiology.

For Acanthamoeba/Naeglaria Culture, send sample to Focus Diagnostics.
Normal range Negative
Critical value Parasites detected from normally sterile sites
Synonyms Amoeba; Amebic meningitis wet mount; Amoebic meningitis wet mount; Amebiasis; Amoebiasis; Amebic meningitis culture; Amoebic meningitis culture
Stability Room temperature 1 day.
Turn around times 1-3 days for Giemsa stain
Additional information For CSF, a wet mount of the concentrated fluid is perfomed and billed separately.

It may be possible to culture Acanthamoeba or Naegleria species, but diagnosis is more rapid by examination of the CSF by Giemsa stain or wet mount with phase contrast microscopy.
For culture, order a Microbiology Special Sendout Test and indicate Acanthamoeba/Naeglaria Culture in the Test Description field.

Prior authorization is required for culture of Brown and Toland patients.
CPT coding
Wet mount 87210
Giemsa stain 87207
Last Updated 11/21/2013 12:42:53 PM
Entry Number 51
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